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Titolo:
Micronucleus induction in Syrian hamster embryo cells following exposure to 50 Hz magnetic fields, benzo(a)pyrene, and TPA in vitro
Autore:
Simko, M; Richard, D; Kriehuber, R; Weiss, DG;
Indirizzi:
Univ Rostock, Inst Cell Biol & Biosyst Technol, Div Environm Physiol, D-18051 Rostock, Germany Univ Rostock Rostock Germany D-18051 m Physiol, D-18051 Rostock, Germany
Titolo Testata:
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
fascicolo: 1-2, volume: 495, anno: 2001,
pagine: 43 - 50
SICI:
1383-5718(20010822)495:1-2<43:MIISHE>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NORMAL HUMAN FIBROBLASTS; BREAST-CANCER MODEL; MOUSE-SKIN MODEL; ELECTROMAGNETIC-FIELDS; TUMOR PROMOTION; WIRING CONFIGURATIONS; DNA-SYNTHESIS; SENCAR MICE; ABERRATIONS; PROTEIN;
Keywords:
carcinogenesis; EMF; SHE cells; micronucleus formation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Simko, M Univ Rostock, Inst Cell Biol & Biosyst Technol, Div Environm Physiol, UnivPl 2, D-18051 Rostock, Germany Univ Rostock Univ Pl 2 Rostock Germany D-18051 Rostock, Germany
Citazione:
M. Simko et al., "Micronucleus induction in Syrian hamster embryo cells following exposure to 50 Hz magnetic fields, benzo(a)pyrene, and TPA in vitro", MUT RES-GTE, 495(1-2), 2001, pp. 43-50

Abstract

Electromagnetic fields (EMFs) have been associated with increased incidence of cancer suggested by epidemiological studies. To test the carcinogenic potency of EMF, the in vitro micronucleus assay with SHE cells has been used as a screening method for genotoxicity. A50 Hz magnetic field (MF) of 1 mT field strength was applied either alone or with the tumour initiator benzo(a)pyrene (BP) or the tumour promoter 12-O-tetradecanoylphorbol-13-acetate(TPA). All three treatments were applied in single, double or triple treatment regimes. MF or TPA (1 nM) alone did not affect the number of micronuclei (MN) in initiated and non-initiated SHE cells. Changing the schedule of the typical initiation protocol, namely applying the initiator (BP) during exposure to NM, results in an 1.8-fold increased MN formation compared to BP treatment alone. Combined experiment with BP, TPA and MF did not cause further MN formation. Since initiation during MY exposure caused a significant increased MN formation, our findings suggest that MFs enhance the initiation process of BP. We think that this MF-enhanced co-carcinogenic effect iscaused by an indirect "cell activation" process. The resulting genomic instability is proposed to be due to free radicals and/or to the unscheduled "switching-on" of signal transduction pathways. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:29:08