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Titolo:
High incidence of mosaic mutations induced by irradiating paternal germ cells of the medaka fish, Oryzias latipes
Autore:
Shimada, A; Shima, A;
Indirizzi:
Univ Tokyo, Sch Sci, Dept Biol Sci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Biol Sci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 d Biosci, Bunkyo Ku, Tokyo 1130033, Japan
Titolo Testata:
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
fascicolo: 1-2, volume: 495, anno: 2001,
pagine: 33 - 42
SICI:
1383-5718(20010822)495:1-2<33:HIOMMI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
JAPANESE MEDAKA; IONIZING-RADIATION; TEST SYSTEM; LOCUS TEST; MUTAGENESIS; INSTABILITY;
Keywords:
mosaic mutations; medaka; male germ cells; gamma-rays;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Shimada, A Univ Tokyo, Sch Sci, Dept Biol Sci, Bunkyo Ku, Hongo 7-3-1, Tokyo 1130033,Japan Univ Tokyo Hongo 7-3-1 Tokyo Japan 1130033 Tokyo 1130033,Japan
Citazione:
A. Shimada e A. Shima, "High incidence of mosaic mutations induced by irradiating paternal germ cells of the medaka fish, Oryzias latipes", MUT RES-GTE, 495(1-2), 2001, pp. 33-42

Abstract

Delayed-type mutations induced by radiation have recently been demonstrated in various somatic-cell systems. Such mutations are thought to result from the transmission of genetic instability through many cell divisions subsequent to a single exposure to ionizing radiation. Here, we have examined whether 'transgenerational' delayed-type mutations can arise during embryonicdevelopment of the medaka fish as a result of exposing the sperm and spermatids of live fish to 137 Cs gamma -radiation. To do this, we made use of asensitive specific-locus test (SLT) for the medaka that we have recently developed. Because the medaka has a transparent egg membrane and embryo body, both visible mosaics and whole-body mutations can be detected during development at an early-expressed pigmentation locus. When wild-type +/+ males were gamma -irradiated and then mated with wl/wl females, the frequency of F1 embryos with both wild-type orange leucophores (wl/+) and mutant-type white leucophores, (wl/wl*) (mosaic mutants) was about 5.7 x 10(-3)/Gy. The frequency of embryos with only white leucophores (whole-body mutants) was about 1.3 x 10(-3)/Gy. These results suggest that delayed mutations frequently arise in medaka fish embryos that have been fertilized with irradiated sperm. Some possible mechanisms involved in the generation of these delayed mutational events (including genomic instability in the early embryos) are discussed. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:28:10