Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Suppression of cellular invasion by activated G-protein subunits G alpha o, G alpha i1, G alpha i2, and G alpha i3 and sequestration of G beta gamma
Autore:
Faivre, S; Regnauld, K; Bruyneel, E; Nguyen, QD; Mareel, M; Emami, S; Gespach, C;
Indirizzi:
Hop St Antoine, INSERM, U482, Unite Signal Transduct & Cellular Funct Diabet &, F-75571 Paris 12, France Hop St Antoine Paris France 12 Funct Diabet &, F-75571 Paris 12, France State Univ Ghent, Expt Cancerol Lab, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 ancerol Lab, B-9000 Ghent, Belgium
Titolo Testata:
MOLECULAR PHARMACOLOGY
fascicolo: 2, volume: 60, anno: 2001,
pagine: 363 - 372
SICI:
0026-895X(200108)60:2<363:SOCIBA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR ACTIVATION; ACTIN CYTOSKELETON; EPITHELIAL-CELLS; RGS PROTEINS; KINASE; SRC; BINDING; RHO; INVASIVENESS; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Gespach, C Hop St Antoine, INSERM, U482, Unite Signal Transduct & CellularFunct Diabet &, 184 Rue Faubourg St Antoine, F-75571 Paris 12, France Hop St Antoine 184 Rue Faubourg St Antoine Paris France 12 nce
Citazione:
S. Faivre et al., "Suppression of cellular invasion by activated G-protein subunits G alpha o, G alpha i1, G alpha i2, and G alpha i3 and sequestration of G beta gamma", MOLEC PHARM, 60(2), 2001, pp. 363-372

Abstract

It was shown previously that platelet-activating factor receptors (PAF-Rs)inhibit invasiveness of colonic and kidney epithelial cells induced by thesrc and Met oncogenes via a pertussis toxin-sensitive mechanism. Therefore, Madin-Darby canine kidney (MDCKts.src) cells were stably transfected withconstitutively activated forms of G alphao, G alpha i1, G alpha i2, G alpha i3 (AG alphao/i), two G beta gamma sequestering proteins [C-terminal end of beta -adrenergic receptor kinase (ct-beta ARK) and the Gat subunit of retinal G-protein transducin], and G beta1-G gamma2 subunits alone or in combination. Cellular invasion induced by src, Met, and leptin was abrogated bythe AG alphao/i, ct-beta ARK, and Gat-positive clones, but was induced by coexpression of G alpha1 gamma2. In contrast, invasion stimulated by the trefoil factors (TFFs) pS2 and intestinal trefoil factor in MDCKts.src cells or human colonic epithelial cells PCmsrc and HCT8/S11 was insensitive to PAF, AG alphao, AG alpha i1, and AG alpha i2, but was abolished by AG alpha i3 and the protease-activated receptor-1 (PAR-1) agonist thrombin receptor-activating peptide. Depletion of free G beta gamma heterodimers by ct-beta ARK resulted in a remarkable decrease of cellular adhesion and spreading on collagen matrix. Our data demonstrate the following: 1) PAF-Rs impair cellular invasion induced by src, Met, and leptin via the activation of G alphaoand G alpha i1 to -3; 2) invasion induced by TFFs is selectively inhibitedby PAR-1 receptors and G alpha i3 activation; and 3) G beta gamma dimers are required as positive effectors of invasion pathways induced by oncogenesand epigenetic factors. Thus, redistribution of G alphao/G alphai and G beta/gamma heterotrimeric G-proteins by PAF-R and PAR-1 exert differential functions on positive and negative signaling pathways involved in cellular invasion and may serve as potential targets for anticancer therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 05:36:23