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Titolo:
Fine genetic mapping and BAC contig development for the citrus tristeza virus resistance gene locus in Poncirus trifoliata (Raf.)
Autore:
Deng, Z; Huang, S; Ling, P; Yu, C; Tao, Q; Chen, C; Wendell, MK; Zhang, HB; Gmitter, FG;
Indirizzi:
Univ Florida, Ctr Citrus Res & Educ, Lake Alfred, FL 33850 USA Univ Florida Lake Alfred FL USA 33850 s & Educ, Lake Alfred, FL 33850 USA Texas A&M Univ, Dept Soil & Crop Sci, Crop Biotechnol Ctr, College Stn, TX77843 USA Texas A&M Univ College Stn TX USA 77843 nol Ctr, College Stn, TX77843 USA
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 4, volume: 265, anno: 2001,
pagine: 739 - 747
SICI:
1617-4615(200106)265:4<739:FGMABC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTIFICIAL CHROMOSOME LIBRARY; PLANT-DISEASE RESISTANCE; NUCLEOTIDE-BINDING SITE; PATHOGEN RESISTANCE; NEMATODE RESISTANCE; LINKAGE MAP; CLONING; MARKERS; CLONES; IDENTIFICATION;
Keywords:
citrus tristeza virus (CTV); resistance gene; genetic mapping; BAC contig development; chromosome landing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Gmitter, FG Univ Florida, Ctr Citrus Res & Educ, 700 Expt Stn Rd, Lake Alfred, FL 33850 USA Univ Florida 700 Expt Stn Rd Lake Alfred FL USA 33850 3850 USA
Citazione:
Z. Deng et al., "Fine genetic mapping and BAC contig development for the citrus tristeza virus resistance gene locus in Poncirus trifoliata (Raf.)", MOL GENET G, 265(4), 2001, pp. 739-747

Abstract

A map-based cloning strategy has been employed to isolate Oil, a single dominant gene from Poncirus trifoliata that confers resistance to citrus tristeza virus (CTV), the most important viral pathogen of citrus. Cloning of this gene will allow development of commercially acceptable, virus-resistantcultivars. A high-resolution genetic linkage map of the Ctv locus region was developed using a backcross population of 678 individuals. Three DNA markers that were closely linked or co-segregated with Ctv were identified andused to screen BAC libraries derived from an intergeneric hybrid of Poncirus and Citrus. Through chromosome walking and landing, two BAC contigs weredeveloped: one encompassing the Cti, region, and the other spanning the allelic susceptibility gene region. The resistance gene contig consists of 20BAC clones and is approximately 550 kb in length; the susceptibility gene contig consists of 16 BAC clones and extends about 450 kb. The Cti, locus was localized within a genomic region of approximately 180 kb by genetic mapping of BAC insert ends. The BAC contigs were integrated with the genetic map; variation in the ratio of genetic to physical distance was observed in the vicinity of Ctv. Southern hybridization data indicated that a few copies of NBS-LRR class sequences are distributed at or around the Cti, locus. Efforts are being made to assign the Ctv locus to a smaller genomic fragmentwhose function can be confirmed through genetic complementation of a CTV susceptible phenotype. These results indicate that map-based gene cloning isfeasible in a woody perennial.

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Documento generato il 31/03/20 alle ore 05:06:38