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Titolo:
Crypt1, an active Ac-like transposon from the chestnut blight fungus, Cryphonectria parasitica
Autore:
Linder-Basso, D; Foglia, R; Zhu, P; Hillman, BI;
Indirizzi:
Rutgers State Univ, Cook Coll, Dept Plant Pathol, New Brunswick, NJ 08901 USA Rutgers State Univ New Brunswick NJ USA 08901 New Brunswick, NJ 08901 USA
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 4, volume: 265, anno: 2001,
pagine: 730 - 738
SICI:
1617-4615(200106)265:4<730:CAAATF>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
POPULATION-STRUCTURE; TOLYPOCLADIUM-INFLATUM; ENDOTHIA-PARASITICA; FUSARIUM-OXYSPORUM; RNA ELEMENT; DIVERSITY; RESTLESS; RECOMBINATION; DROSOPHILA; DSRNA;
Keywords:
filamentous fungi; transposon; chestnut blight; population biology; fungal virus; Cryphonectria parasitica;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Hillman, BI Rutgers State Univ, Cook Coll, Dept Plant Pathol, Foran Hall,59 Dudley Rd,New Brunswick, NJ 08901 USA Rutgers State Univ Foran Hall,59 Dudley Rd New Brunswick NJ USA 08901
Citazione:
D. Linder-Basso et al., "Crypt1, an active Ac-like transposon from the chestnut blight fungus, Cryphonectria parasitica", MOL GENET G, 265(4), 2001, pp. 730-738

Abstract

A moderately repetitive element was identified previously in the nuclear genome of the chestnut blight fungus, Cryphonectria parasitica, and has beenused as a probe for population studies of the fungus. We report here that the repetitive element is a class Il transposon of the hAT family of Activator (Ac)-like transposable elements. The element, named Crypt1, has a size of 3563 bp, including 21-bp terminal inverted repeats. A unique 8-bp directrepeat sequence flanking Crypt1 was identified in each of three clones examined. A single large ORF with the potential to encode a putative transposase of 946 amino acid residues was deduced from the sequence of Crypt1. Based on amino acid sequence alignments, Crypt1 is most closely related to other Ac-like transposons of filamentous ascomycetes. A single transcript of approximately 3.0 kb was identified by Northern hybridization experiments from Crypt1-containing isolates, suggesting that Crypt1 is an active element. An isolate containing a single, possibly defective, copy of Crypt1 was identified in C. parasitica isolates from China; no Crypt1 transcript was identified in this isolate. Transposition of Crypt1 was inferred from Southern and inverse PCR analyses of C. parasitica isolates maintained in the laboratory, but transposition appears to be a rare event.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 10:11:51