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Titolo:
Analysis of the introgression of Solanum bulbocastanum DNA into potato breeding lines
Autore:
Naess, SK; Bradeen, JM; Wielgus, SM; Haberlach, GT; McGrath, JM; Helgeson, JP;
Indirizzi:
Univ Wisconsin, Dept Plant Pathol, USDA ARS, Plant Dis Resistance Res Unit, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 tance Res Unit, Madison, WI 53706 USA
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 4, volume: 265, anno: 2001,
pagine: 694 - 704
SICI:
1617-4615(200106)265:4<694:AOTIOS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOMATIC HYBRIDS; RFLP ANALYSIS; PHYTOPHTHORA-INFESTANS; LATE BLIGHT; QUANTITATIVE RESISTANCE; LINKAGE MAP; TUBEROSUM; TOMATO; MARKERS; BREVIDENS;
Keywords:
somatic hybrid; potato; Solanum bulbocastanum; RFLP; RAPD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Helgeson, JP Univ Wisconsin, Dept Plant Pathol, USDA ARS, Plant Dis Resistance Res Unit, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 t, Madison, WI 53706 USA
Citazione:
S.K. Naess et al., "Analysis of the introgression of Solanum bulbocastanum DNA into potato breeding lines", MOL GENET G, 265(4), 2001, pp. 694-704

Abstract

Somatic hybrids have been obtained between potato and Solanum bulbocastanum PI 245310, a Mexican diploid (2n=2x=24) species. Through restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD) analyses it was found that the somatic hybrids contain each chromosome of the diploid parent and that the synteny of RFLP markers noted with tomato, potato and S. brevidens is largely maintained in S. bulbocastanum. RFLP analyses of BC I progeny of two different hybrids indicated that a substantial number of markers were either lost or were heterozygous, in marked contrast with results previously noted with S. brevidens. A RAPD map for all 12 chromosomes of S. bulbocastanum was prepared and marker transmission was followed in three BC2 populations. Results with chromosomes 3, 8 and 10 from these populations are compared.

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Documento generato il 31/05/20 alle ore 13:07:50