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Titolo:
The 5 ' terminal region of the Schizosaccharomyces pombe mes1 mRNA is crucial for its meiosis-specific splicing
Autore:
Shimoseki, M; Shimoda, C;
Indirizzi:
Osaka City Univ, Grad Sch Sci, Dept Biol, Sumiyoshi Ku, Osaka 5588585, Japan Osaka City Univ Osaka Japan 5588585 , Sumiyoshi Ku, Osaka 5588585, Japan
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 4, volume: 265, anno: 2001,
pagine: 673 - 682
SICI:
1617-4615(200106)265:4<673:T5'TRO>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
FISSION YEAST; SACCHAROMYCES-CEREVISIAE; TRANSCRIPTION FACTOR; GENETIC-ANALYSIS; U1 SNRNP; RNA; SEQUENCE; VECTORS; PROTEIN; BINDING;
Keywords:
Schizosaccharomyces pombe; splicing; meiosis; mes1; fission yeast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Shimoda, C Osaka City Univ, Grad Sch Sci, Dept Biol, Sumiyoshi Ku, Osaka 5588585, Japan Osaka City Univ Osaka Japan 5588585 Ku, Osaka 5588585, Japan
Citazione:
M. Shimoseki e C. Shimoda, "The 5 ' terminal region of the Schizosaccharomyces pombe mes1 mRNA is crucial for its meiosis-specific splicing", MOL GENET G, 265(4), 2001, pp. 673-682

Abstract

The mes1(+) gone of Schizosaccharomyces pombe is required for the second meiotic division. The single 75-nt intron in mes1 is spliced out only in meiotic cells. Here we report a cis-acting element which is responsible for meiosis-specific splicing. Both 5' and 3' splice sites of the mes1 intron deviate from the consensus sequence. Point mutations which altered these sitesso that they conformed to the consensus, however, did not affect the splicing pattern of mes1. Neither replacement of the mes1 intron with the constitutively spliced intron of the nda3 gene, nor replacement of the 3' exon with E. coli lacZ changed the splicing pattern. In contrast, deletion of the 5' terminal 125 nt from the 5' exon derepressed splicing in vegetative cells, implying that this 5' terminal sequence, named SRE (mes1 splicing repression element), inhibits splicing of the downstream intron. A potential stem-loop structure in the SRE is predicted. Disruption of this stem structure by mutation abolished the repression of mes1 splicing in vegetative cells. Overexpression of the SRE sequence on a multicopy plasmid also relieved therepression of splicing of the authentic mes1 transcripts. These results suggest that as yet unknown trans-acting factors inhibit splicing of the mes1transcript in vegetative cells by interacting with the cis-element SRE.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 04:11:07