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Titolo:
Structural and functional characterization of the recR gene of Streptomyces
Autore:
Pelaez, AI; Ribas-Aparicio, RM; Gomez, A; Rodicio, MR;
Indirizzi:
Univ Oviedo, Inst Univ Biotecnol, Area Microbiol, Dept Biol Fonct, E-33006Oviedo, Spain Univ Oviedo Oviedo Spain E-33006 , Dept Biol Fonct, E-33006Oviedo, Spain Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City, DF, Mexico Inst Politecn Nacl Mexico City DF Mexico robiol, Mexico City, DF, Mexico
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 4, volume: 265, anno: 2001,
pagine: 663 - 672
SICI:
1617-4615(200106)265:4<663:SAFCOT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINATIONAL DNA-REPAIR; ESCHERICHIA-COLI; SEQUENCE-ANALYSIS; CLONING VECTORS; MOLECULAR-CLONING; BACILLUS-SUBTILIS; REPLICATION FORKS; CHROMOSOMAL DNA; PHYSICAL MAP; LIVIDANS;
Keywords:
Streptomyces coelicolor; Streptomyces lividans; recR; recombinational DNA repair; genetic complementation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Rodicio, MR Univ Oviedo, Inst Univ Biotecnol, Area Microbiol, Dept Biol Fonct, E-33006Oviedo, Spain Univ Oviedo Oviedo Spain E-33006 Fonct, E-33006Oviedo, Spain
Citazione:
A.I. Pelaez et al., "Structural and functional characterization of the recR gene of Streptomyces", MOL GENET G, 265(4), 2001, pp. 663-672

Abstract

The recR gene product is necessary for homologous recombination and recombinational DNA repair in eubacteria. We report the isolation and sequencing of the recR gene from Streptomyces coelicolor. It encodes a protein of 198 amino acids, with a predicted molecular mass of 22 kDa. The deduced amino acid sequence shows significant similarity to that of RecR proteins from other bacteria, including Escherichia coli and Bacillus subtilis. Like these, Streptomyces RecR contains potential helix-hairpin-helix, zinc finger and ATP-binding motifs, as well as the Toprim domain which is present also in topoisomerases of Types IA and II, primases and nucleases of the OLD family. The recR genes of Escherichia coli and Bacillus subtilis are immediately preceded by a small ORF (orf12 and orf107, respectively). An equivalent ORF (orf1) is also found in Streptomyces. S. lividans recR mutants, obtained either by insertional inactivation of recR or by deletion of the gene togetherwith the preceding ORF, displayed increased sensitivity to DNA-damaging agents (such as UV light and methylmethanesulfonate), when compared with the wild-type strain. Both mutants could be complemented by the wild-type orf1recR genes and also by the recR gene alone. Based on these results, orf1 appears to be dispensable for the repair function of Streptomyces RecR. In studies of heterologous complementation, the B. subtilis recR region (orf107recR) was found to complement the S. lividans Delta orf1recR mutant, but the equivalent region from E. coli (orf12recR) could not. However. in the absence of orf107, B. subtilis recR was unable to restore the wild-type phenotype to the Streptomyces deletion mutant.

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Documento generato il 06/07/20 alle ore 03:12:39