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Titolo:
A mutation in SPC42, which encodes a component of the spindle pole body, results in production of two-spored asci in Saccharomyces cerevisiae
Autore:
Ishihara, S; Hirata, A; Minemura, M; Nogami, S; Ohya, Y;
Indirizzi:
Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 d Biosci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 r Biosci, Bunkyo Ku, Tokyo 1130033, Japan Japan Sci & Technol Corp, PRESTO, Unit Proc & Combined Circuit, Toyonaka, Osaka 5650082, Japan Japan Sci & Technol Corp Toyonaka Osaka Japan 5650082saka 5650082, Japan
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 4, volume: 265, anno: 2001,
pagine: 585 - 595
SICI:
1617-4615(200106)265:4<585:AMISWE>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPB DUPLICATION; GENE-EXPRESSION; YEAST; MEIOSIS; SPORULATION; RECOMBINATION; PLAQUES; NDT80; DNA;
Keywords:
meiosis; prospore membrane formation; SPC42; spindle pole body; Saccharomyces cerevisiae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Ohya, Y Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 , Bunkyo Ku, Tokyo 1130033, Japan
Citazione:
S. Ishihara et al., "A mutation in SPC42, which encodes a component of the spindle pole body, results in production of two-spored asci in Saccharomyces cerevisiae", MOL GENET G, 265(4), 2001, pp. 585-595

Abstract

In the yeast Saccharomyces cerevisiae, SPC42 is an essential gene, which encodes one of the major components of the spindle pole body (SPB). We report on a mutation in the SPC42 gene (spc42-102) that results in a sporulation-specific defect. Mitotic growth of haploid and diploid spc42-102 strains is normal and both exhibit the same growth rates as the isogenic wild-type strains. Many diploid spc42-102/spc42-102 cells undergo normal meiotic nuclear divisions, producing four haploid nuclei. However, a significant fraction of meiotic spc42-102/spc42-102 cells contain two immature SPBs and aberrant nuclei that are not surrounded by a prospore membrane. Some 40% of the resultant asci contain only two spores, while wild-type diploid cells almostalways produce four-spored asci. Segregation of auxotrophic markers that are tightly linked to the centromere reveals that two-spore asci formed fromspc42-102/spc42-102 diploid cells exclusively contain nonsister haploid spores. Western analysis and measurements of the fluorescent signal from an Spc42p-GFP (green fluorescent protein) fusion reveal that the mutant strain fails to accumulate Spc42p at meiosis. Thus, our results suggest that insufficiency of Spc42p during meiosis results in a pair of immature nonsister SPBs that are not enclosed by prospore membrane.

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Documento generato il 30/03/20 alle ore 19:40:19