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Titolo:
Multiple promoters exist in the human GR gene, one of which is activated by glucocorticoids
Autore:
Breslin, MB; Geng, CD; Vedeckis, WV;
Indirizzi:
Louisiana State Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, New Orleans, LA 70112 USA Louisiana State Univ New Orleans LA USA 70112 , New Orleans, LA 70112 USA Louisiana State Univ, Hlth Sci Ctr, Stanley S Scott Canc Ctr, New Orleans,LA 70112 USA Louisiana State Univ New Orleans LA USA 70112 r, New Orleans,LA 70112 USA
Titolo Testata:
MOLECULAR ENDOCRINOLOGY
fascicolo: 8, volume: 15, anno: 2001,
pagine: 1381 - 1395
SICI:
0888-8809(200108)15:8<1381:MPEITH>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LYMPHOBLASTIC-LEUKEMIA; LYMPHOID-CELL LINE; RECEPTOR MESSENGER-RNA; TRANSCRIPTIONAL ACTIVATOR; REGULATORY ELEMENTS; DNA-SEQUENCES; EXPRESSION; IRF-1; CHILDHOOD; IFN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Vedeckis, WV Louisiana State Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, 533 Bolivar St, New Orleans, LA 70112 USA Louisiana State Univ 533 Bolivar St New Orleans LA USA 70112
Citazione:
M.B. Breslin et al., "Multiple promoters exist in the human GR gene, one of which is activated by glucocorticoids", MOL ENDOCR, 15(8), 2001, pp. 1381-1395

Abstract

A new human GR gene sequence (hGR 1Ap/e), which is distinct from the previously identified human GR promoter and coding sequences, has been isolated and characterized. The hGR 1Ap/e sequence is approximately 31 kbp upstream of the human GR coding sequence. This sequence (2,056 bp) contains a novel promoter (the hGR 1A promoter; 1,075 bp) and untranslated exon sequence (hGR exon 1A sequence; 981 bp). Alternative splicing produces three different hGR 1A-containing transcripts, 1A1, 1A2, and 1A3. GR transcripts containingexon 1 All, 1A2, 1B, and IC are expressed at various levels in many cancercell lines, while the exon 1A3-containing GR transcript is expressed most abundantly in blood cell cancer cell lines. Glucocorticoid hormone treatment causes an upregulation of exon 1A3-containing GR transcripts in CEM-C7 T-lymphoblast cells and a down-regulation of exon 1A3-containing transcripts in IM-9 B-lymphoma cells. Deoxyribonuclease I footprinting using CEM-C7 cell nuclear extract reveals four footprints in the promoter region and two intraexonic footprints. Much of the basal promoter-activating function is found in the +41/+269 sequence, which contains two deoxyribonuclease I footprints (FP5 and FP6). When this sequence is cloned into the pXP-1 luciferase reporter gene, hormone treatment causes a significant increase in luciferaseactivity in Jurkat T cells that are cotransfected with a GR expression vector. FP5 is an interferon regulatory factor-binding element, and it contributes significantly to basal transcription rate, but it is not activated by steroid. FP6 resembles a glucocorticoid response element and can bind GR beta. This novel hGR 1Ap/e sequence may have future applications for the diagnosis, prognosis, and treatment of T-cell leukemia and lymphoma.

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Documento generato il 25/01/20 alle ore 15:56:46