Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Heat shock interferes with steroidogenesis by reducing transcription of the steroidogenic acute regulatory protein gene
Autore:
Murphy, BD; Lalli, E; Walsh, LP; Liu, ZM; Soh, J; Stocco, DM; Sassone-Corsi, P;
Indirizzi:
Univ Montreal, Ctr Rech & Reprod Anim, St Hyacinthe, PQ J2S 7C6, Canada Univ Montreal St Hyacinthe PQ Canada J2S 7C6 yacinthe, PQ J2S 7C6, Canada Texas Tech Univ, Hlth Sci Ctr, Dept Cell Biol & Biochem, Lubbock, TX 79430USA Texas Tech Univ Lubbock TX USA 79430 Biol & Biochem, Lubbock, TX 79430USA ULP, INSERM, Ctr Natl Rech Sci, Inst Genet & Biol Mol Cellulaire, F-67404 Illkirch, France ULP Illkirch France F-67404 iol Mol Cellulaire, F-67404 Illkirch, France
Titolo Testata:
MOLECULAR ENDOCRINOLOGY
fascicolo: 8, volume: 15, anno: 2001,
pagine: 1255 - 1263
SICI:
0888-8809(200108)15:8<1255:HSIWSB>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; LEYDIG TUMOR-CELLS; RAT LUTEAL CELLS; STIMULATING-HORMONE; PROMOTER ACTIVITY; MAMMALIAN-CELLS; STAR GENE; EXPRESSION; MULTIPLE; CHOLESTEROL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Murphy, BD Univ Montreal, Ctr Rech & Reprod Anim, 3200 Rue Sicotte, St Hyacinthe, PQ J2S 7C6, Canada Univ Montreal 3200 Rue Sicotte St Hyacinthe PQ Canada J2S 7C6 a
Citazione:
B.D. Murphy et al., "Heat shock interferes with steroidogenesis by reducing transcription of the steroidogenic acute regulatory protein gene", MOL ENDOCR, 15(8), 2001, pp. 1255-1263

Abstract

A key regulatory point in fine tuning of steroidogenesis is the synthesis of steroidogenic acute regulatory protein, which transfers cholesterol intomitochondria. Heat shock and toxic insults reduce steroidogenic acute regulatory protein, severely compromising steroid synthesis. As the molecular mechanisms for this reduction remain elusive, we tested the hypothesis that heat shock directly interferes with transcription of the steroidogenic acute regulatory protein gene. We show that, in mouse MA-10 Leydig tumor cells,heat shock causes drastic declines in (Bu)(2)cAMP-induced progesterone accumulation and steroidogenic acute regulatory protein transcript abundance. A proximal steroidogenic acute regulatory protein promoter fragment (-85 to+39) is sufficient to direct both cAMP inducibility and heat shock inhibition. Nuclear extracts from MA-10 cells displayed binding to this proximal promoter fragment as a low mobility complex in gel shift experiments. This complex disappeared in nuclear extracts taken at 5 and 10 min after initiation of heat shock and reappeared in extracts taken at 2 and 8 h. Similar low-mobility complexes formed on oligonucleotides representing the overlappingsubfragments of the minimal steroidogenic acute regulatory protein promoter fragment sensitive to the heat shock effect. Extracts from heat-shocked MA-10 cells displayed reduced complex formation to each of the subfragments. We conclude that heat shock reduces progesterone synthesis, steroidogenic acute regulatory protein mRNA abundance, and activity and disrupts binding of nuclear proteins to the proximal region of the steroidogenic acute regulatory protein promoter. Together these observations provide strong evidencefor a mechanism of transcriptional inhibition in the down-regulation of steroidogenic acute regulatory protein expression by heat shock.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 07:37:21