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Titolo:
The effect of type I astrocytes on neuronal complexity: A fractal analysis
Autore:
Schaffner, AE; Ghesquiere, A;
Indirizzi:
NINDS, Neurophysiol Lab, NIH, Bethesda, MD 20892 USA NINDS Bethesda MD USA 20892 Neurophysiol Lab, NIH, Bethesda, MD 20892 USA
Titolo Testata:
METHODS
fascicolo: 4, volume: 24, anno: 2001,
pagine: 323 - 329
SICI:
1046-2023(200108)24:4<323:TEOTIA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVITY-DEPENDENT DEVELOPMENT; RAT HIPPOCAMPAL-NEURONS; SPINAL-CORD; NEURITE OUTGROWTH; CELLS; GLIA; DIFFERENTIATION; VITRO; MORPHOLOGY; MODULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Schaffner, AE Ctr Sci Review, 6701 Rockledge Dr,Room 5214 MSC 7850, Bethesda, MD 20892 USA Ctr Sci Review 6701 Rockledge Dr,Room 5214 MSC 7850 Bethesda MD USA 20892
Citazione:
A.E. Schaffner e A. Ghesquiere, "The effect of type I astrocytes on neuronal complexity: A fractal analysis", METHODS, 24(4), 2001, pp. 323-329

Abstract

Embryonic, ventral spinal cord neurons were grown on ploy(D-lysine) (PDL) or on a monolayer of type I astrocytes. At various times from 6 h to 2 weeks postplating, cells were fluorescently labeled and fixed with 4% paraformaldehyde. The cell surface immunoreaction allowed visualization of neurons in their entirety, namely, cell bodies and various membranous extensions that included lamellipodia, growth cones, axons, and dendrites. Outlines were drawn for individual neurons and their fractal dimension (D) was calculated. Neurons on poly(D-lysine) reached a peak D at 3 days in vitro, 1 day later than neurons on astrocytes (2 days in vitro). The maximum D was greater for cells on poly(D-lysine) when compared with neurons on astrocytes. In a second experiment the maximum D was similar for neurons on both surfaces butneurons on PDL maintained a higher D for a much longer period than neuronson astrocytes. An examination of fluorescent images revealed that neurons on poly(D-lysine) exhibited lamellipodia and large growth cones for severaldays and these structures were likely responsible for the high D seen in these cells. These structures were rarely observed in neurons plated on astrocytes. Interestingly, D on both surfaces decreased to a similar value at between :1 and 2 weeks in vitro. The trend for D in these cultures, an initial increase to a peak value followed by a decrease to a stable value, is discussed in light of the chemical nature of the two surfaces and synapse formation and stabilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 12:23:50