Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Differentiation of embryonic stem cell-derived dopaminergic neurons is enhanced by survival-promoting factors
Autore:
Rolletschek, A; Chang, H; Guan, KM; Czyz, J; Meyer, M; Wobus, AM;
Indirizzi:
Inst Plant Genet & Crop Plant Res, In Vitro Differentiat Grp, D-06466 Gatersleben, Germany Inst Plant Genet & Crop Plant Res Gatersleben Germany D-06466 n, Germany Univ So Denmark, Med Biol Inst, DK-5000 Odense C, Denmark Univ So DenmarkOdense Denmark C ed Biol Inst, DK-5000 Odense C, Denmark
Titolo Testata:
MECHANISMS OF DEVELOPMENT
fascicolo: 1-2, volume: 105, anno: 2001,
pagine: 93 - 104
SICI:
0925-4773(200107)105:1-2<93:DOESCD>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; IN-VITRO DIFFERENTIATION; PRIMORDIAL GERM-CELLS; NEUROTROPHIC FACTOR; PROGENITOR CELLS; PRECURSOR CELLS; MESSENGER-RNA; CARDIAC DIFFERENTIATION; TYROSINE-HYDROXYLASE; SUBSTANTIA-NIGRA;
Keywords:
embryonic stem cell differentiation; dopaminergic neuron; tyrosine hydroxylase; Nurr1; interleukin-1 beta glial cell line-derived neurotrophic factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Wobus, AM Inst Plant Genet & Crop Plant Res, In Vitro Differentiat Grp, D-06466 Gatersleben, Germany Inst Plant Genet & Crop Plant Res Gatersleben Germany D-06466
Citazione:
A. Rolletschek et al., "Differentiation of embryonic stem cell-derived dopaminergic neurons is enhanced by survival-promoting factors", MECH DEVEL, 105(1-2), 2001, pp. 93-104

Abstract

Here, we describe the generation of viable and dopamin-producing neurons derived from pluripotent mouse embryonic stem cells. Neurotrophic factors incombination with survival-promoting factors, such as interleukin-1 beta, glial cell line-derived neurotrophic factor, neurturin, transforming growth factor-beta (3) and dibutyryl-cyclic AMP, significantly enhanced Nurr1 and tyrosine hydroxylase (TH) mRNA levels, whereas En-1, mash-1 and dopamine-2-receptor mRNA levels were not upregulated. In parallel, mRNA levels of the anti-apoptotic gene bcl-2 were found to be upregulated at terminal stages. Double immunofluorescence analysis revealed increased numbers of TH- and dopamine transporter-, but not gamma -aminobutyric acid- and serotonin-positive neurons in relation to synaptophysin-labeled cells by survival-promotingfactors. Moreover, high-performance liquid chromatography analysis showed detectable levels of intracellular dopamine. We conclude that survival-promoting factors enhance differentiation, survival and maintenance of dopaminergic neurons derived from embryonic stem cells. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 01:13:59