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Titolo:
Gene trap insertion reveals two open reading frames in the mouse SSeCKS gene: the form predominantly detected in the nervous system is suppressed by the insertion while the other, specific of the testis, remains expressed
Autore:
Camus, A; Mesbah, K; Rallu, M; Babinet, C; Barra, J;
Indirizzi:
Inst Pasteur, CNRS, URA 1960, Dev Biol Unit, F-75724 Paris 15, France InstPasteur Paris France 15 60, Dev Biol Unit, F-75724 Paris 15, France
Titolo Testata:
MECHANISMS OF DEVELOPMENT
fascicolo: 1-2, volume: 105, anno: 2001,
pagine: 79 - 91
SICI:
0925-4773(200107)105:1-2<79:GTIRTO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINASE-C SUBSTRATE; PROTEIN-KINASE; ANCHORING PROTEINS; BINDING PROTEIN; FIBROUS SHEATH; CELLS; LOCALIZATION; SPERM; PKA; SRC;
Keywords:
SSeCKS gene; A-kinase anchoring protein; scaffold protein; gene trap; mouse development; LacZ reporter gene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Barra, J Inst Pasteur, CNRS, URA 1960, Dev Biol Unit, 25 Rue Dr Roux, F-75724 Paris15, France Inst Pasteur 25 Rue Dr Roux Paris France 15 5724 Paris15, France
Citazione:
A. Camus et al., "Gene trap insertion reveals two open reading frames in the mouse SSeCKS gene: the form predominantly detected in the nervous system is suppressed by the insertion while the other, specific of the testis, remains expressed", MECH DEVEL, 105(1-2), 2001, pp. 79-91

Abstract

Scaffold proteins play an important role in regulating signal transductionby targeting kinases and phosphatases in close proximity to their relevantsubstrates. SSeCKS protein has been described as a protein kinase C and A (PKC/PKA) anchoring protein as well as a PKC substrate with a tumor suppressor activity. In this study, we report the generation, via gene trapping inembryonic stem cells of mice carrying an insertion in the mouse SSeCKS gene. Through the molecular analysis of the insertion site, we show that SSeCKS contains two alternative promoters directing the synthesis of mRNAs (P1- and P2-mRNA), encoding two different proteins, one of which would be a truncated form of the other. Interestingly, these RNAs are differentially expressed, P2 being found exclusively in the male germ line, while P1 exhibits adynamic and wider pattern of expression during embryonic development and in the adult; its expression is predominant in the nervous system. Finally, we show that P1- but not P2-mRNA expression is abolished by the insertion and furthermore that mice homozygous for the mutation lack SSeCKS in all tissues except the malt: germ cells. Nevertheless and surprisingly, these micedo not exhibit any obvious phenotype. The functional implications of theseobservations are discussed. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:08:57