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Titolo:
Expression of hepatic transporters OATP-C and MRP2 in primary sclerosing cholangitis
Autore:
Oswald, M; Kullak-Ublick, GA; Paumgartner, G; Beuers, U;
Indirizzi:
Univ Munich, Klinikum Grosshadern, Med Klin 2, D-81377 Munich, Germany Univ Munich Munich Germany D-81377 , Med Klin 2, D-81377 Munich, Germany Univ Hosp, Dept Med, Div Clin Pharmacol Toxicol, Zurich, Switzerland Univ Hosp Zurich Switzerland lin Pharmacol Toxicol, Zurich, Switzerland Univ Hosp, Dept Med, Div Gastroenterol Hepatol, Zurich, Switzerland Univ Hosp Zurich Switzerland Gastroenterol Hepatol, Zurich, Switzerland
Titolo Testata:
LIVER
fascicolo: 4, volume: 21, anno: 2001,
pagine: 247 - 253
SICI:
0106-9543(200108)21:4<247:EOHTOA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
FAMILIAL INTRAHEPATIC CHOLESTASIS; ORGANIC ANION TRANSPORTER; BILE-DUCT LIGATION; SALT EXPORT PUMP; RAT-LIVER; DOWN-REGULATION; GENE; IDENTIFICATION; MEMBRANE;
Keywords:
bile acids and salts; carrier protein; cholestasis; gene expression; organic anion transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Beuers, U Univ Munich, Klinikum Grosshadern, Med Klin 2, Marchioninistr 15, D-81377 Munich, Germany Univ Munich Marchioninistr 15 Munich Germany D-81377 h, Germany
Citazione:
M. Oswald et al., "Expression of hepatic transporters OATP-C and MRP2 in primary sclerosing cholangitis", LIVER, 21(4), 2001, pp. 247-253

Abstract

Background/Aims: In chronic cholestatic liver diseases, biliary excretion of organic anions from blood into bile is impaired. The aim of this study was to identify the underlying mechanism. Methods: Expression of the basolateral organic anion transporting polypeptide OATP-C (SLC21A6) and the canalicular multidrug resistance protein 2 (MRP2) was studied in patients with primary sclerosing cholangitis (PSC) (n=4), a chronic cholestatic liver disease, and in non-cholestatic controls (n=4) (two with chronic hepatitis C, one with idiopathic liver cirrhosis and one with fatty liver). Total RNA was isolated from liver tissue, reverse transcribed and subjected to polymerasechain reaction (PCR) amplification using primers specific for OATP-C, MRP2and beta -actin. PCR products were quantified densitometrically. Results: When normalized for beta -actin expression, the level of OATP-C mRNA in liver tissue of patients with PSC was 49% of controls (OATP-C/beta -actin 1.60/-0.25 vs. 3.24+/-0.69, p<0.05) and the level of MRP2 mRNA was 27% of controls (MRP2/<beta>-actin 0.70+/-0.36 vs. 2.54+/-0.56;p<0.01). Conclusions: Both OATP-C and MRP2 are decreased as measured by mRNA level in PSC. Downregulation of OATP-C might be the consequence of impaired canalicular secretion of organic anions and could serve to reduce the organic anion load of cholestatic hepatocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 05:32:09