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Titolo:
Bcl-2 antisense oligonucleotides enhance the cytotoxicity of Chlorambucil in B-cell chronic lymphocytic leukaemia cells
Autore:
Pepper, C; Hooper, K; Thomas, A; Hoy, T; Bentley, P;
Indirizzi:
Llandough Hosp, Dept Haematol, Penarth CF64 2XX, S Glam, Wales Llandough Hosp Penarth S Glam Wales CF64 2XX arth CF64 2XX, S Glam, Wales
Titolo Testata:
LEUKEMIA & LYMPHOMA
fascicolo: 3, volume: 42, anno: 2001,
pagine: 491 - 498
SICI:
1042-8194(200107)42:3<491:BAOETC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO; LEUKEMIA; APOPTOSIS; EXPRESSION; BAX; RESISTANCE; INDUCTION; SURVIVAL; CHEMORESISTANCE; LYMPHOMA;
Keywords:
Bcl-2; antisense oligonucleotides Cytototoxicity; B-cell CLL; chlorambucil; chronic lymphocytic leukaemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Pepper, C Llandough Hosp, Dept Haematol, Penlan Rd, Penarth CF64 2XX, S Glam, Wales Llandough Hosp Penlan Rd Penarth S Glam Wales CF64 2XX am, Wales
Citazione:
C. Pepper et al., "Bcl-2 antisense oligonucleotides enhance the cytotoxicity of Chlorambucil in B-cell chronic lymphocytic leukaemia cells", LEUK LYMPH, 42(3), 2001, pp. 491-498

Abstract

We have previously shown that the Bcl-2 antisense oligonucleotide ODN 2009can induce apoptosis in B-cell chronic lymphocytic leukaemia (B-CLL) cells. In this study we evaluated whether ODN 2009 could increase the sensitivity of B-CLL cells to Chlorambucil-induced cell death in vitro in order to establish whether the notion of antisense-mediated chemosensitisation could be applied to B-CLL. Bcl-2 antisense in combination with Chlorambucil resulted in a more marked reduction in Bcl-2 protein expression (p = 0.003), enhanced Bax expression (p < 0.0001) and increased apoptosis when compared to cells incubated with Chlorambucil alone (p = 0.03). This increased in vitro cytotoxicity demonstrates a proof of the concept that a combination of Bcl-2 antisense oligonucleotides with conventional chemotherapeutic drugs may elicit an enhanced therapeutic effect in B-CLL and should therefore be considered for further investigation in the form of a clinical trial.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 04:07:21