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Titolo:
Lack of association between N-ras gene mutations and clinical prognosis inBrazilian children with acute lymphoblastic leukemia
Autore:
Clementino, NCD; Yamamoto, M; Viana, MB; Figueiredo, MS; Kerbauy, J; Saad, STO; Costa, FF;
Indirizzi:
Univ Fed Sao Paulo, MD Disciplina Hematol & Hemoterapia, UNIFESP EPM, BR-04023900 Sao Paulo, Brazil Univ Fed Sao Paulo Sao Paulo Brazil BR-04023900BC3900 Sao Paulo, Brazil
Titolo Testata:
LEUKEMIA & LYMPHOMA
fascicolo: 3, volume: 42, anno: 2001,
pagine: 473 - 479
SICI:
1042-8194(200107)42:3<473:LOABNG>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LYMPHOCYTIC-LEUKEMIA; POINT MUTATIONS; POOR-PROGNOSIS; ONCOGENES; CHILDHOOD; DNA;
Keywords:
acute lymphoblastic leukaemia; ALL; oncogene; N-ras gene mutations;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Yamamoto, M Univ Fed Sao Paulo, MD Disciplina Hematol & Hemoterapia, UNIFESP EPM, R Botucatu 740,V Clementino, BR-04023900 Sao Paulo, Brazil Univ FedSao Paulo R Botucatu 740,V Clementino Sao Paulo Brazil BR-04023900 BC
Citazione:
N.C.D. Clementino et al., "Lack of association between N-ras gene mutations and clinical prognosis inBrazilian children with acute lymphoblastic leukemia", LEUK LYMPH, 42(3), 2001, pp. 473-479

Abstract

Point mutations in codons 12, 13 and 61 of the N-ras proto-oncogene have been detected in several human malignancies. We studied 170 patients with acute lymphoblastic leukemia (ALL), treated from 1988 to 1994 according to a protocol derived from BFM-83 studies, in order to evaluate the incidence and prognostic significance of mutations in this gene in childhood ALL. DNA was extracted from bone marrow smears at diagnosis and amplified by polymerase chain reaction (PCR). After screening with SSCP, PCR products were hybridized with allele specific probes and, in some cases, cloned in a pMOS BlueT vector and sequenced. Exon 2 was also studied in 101 children. Our results showed 4% of mutations in codons 12 and 13 and 2% in exon 2. Similar to a previous report, we identified 7% of mutations among children who were studied for both exons. A new mutation in codon 64 of the N-ras gene was detected in one patient. No significant clinical differences between patients with and without mutations were detected (sex, age, leukocyte counts at diagnosis, nutritional status, and risk factor according to the BFM protocol). Children with mutations in codons 12 and 13 showed significantly higher reactivity to PAS staining on blast cells than children with a wild type N-rasgene configuration. Comparison of overall- and recurrence-free survival did not show significant difference between groups with and without mutations. Our results suggest that mutations in the ras gene are infrequent in children with ALL at diagnosis and seem to be of low prognostic value.

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Documento generato il 23/10/20 alle ore 11:19:46