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Titolo:
Autologous chondrocyte implantation: cells phenotype and proliferation analysis
Autore:
Gigante, A; Bevilacqua, C; Zara, C; Travasi, M; Chillemi, C;
Indirizzi:
Univ Ancona, Dept Orthoped, I-60100 Ancona, Italy Univ Ancona Ancona Italy I-60100 a, Dept Orthoped, I-60100 Ancona, Italy Loreto Hosp, Dept Orthoped, Naples, Italy Loreto Hosp Naples ItalyLoreto Hosp, Dept Orthoped, Naples, Italy
Titolo Testata:
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
fascicolo: 4, volume: 9, anno: 2001,
pagine: 254 -
SICI:
0942-2056(200107)9:4<254:ACICPA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTICULAR-CARTILAGE REPAIR; S-100 PROTEIN;
Keywords:
chondrocyte; cartilage repair; cell culture; cytochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Gigante, A Univ Ancona, Dept Orthoped, Largo Cappelli 1, I-60100 Ancona, Italy Univ Ancona Largo Cappelli 1 Ancona Italy I-60100 ncona, Italy
Citazione:
A. Gigante et al., "Autologous chondrocyte implantation: cells phenotype and proliferation analysis", KNEE SURG S, 9(4), 2001, pp. 254

Abstract

The phenotype and proliferation of human chondrocytes in culture were analyzed before they were implanted as autologous graft in cartilage lesions. During ten autologous chondrocyte implantations in articular cartilage lesions of the knee in ten patients, small amounts of cells to be implanted werecollected and analyzed by morphology, cytochemistry (alcian blue, safranin-O), and immunocytochemistry (antibodies anti-S100 protein, anti-collagen types I and II, anti-chondroitin-S). In four cases the cells were also cultured for 3 weeks. At 1, 10, and 20 days of culture cells were incubated with1 mu Ci/ml [H-3]thymidine for proliferation analysis. In all cases the cells showed the morphological appearance of mature chondrocytes, stained positively for alcian blue and safranin-O, and revealed a strong immunoreactionfor S-100 protein, type II collagen, and chondroitin-S but not for type I collagen. Radioisotope assay of chondrocyte proliferation at 1, 10, and 20 days of culture revealed a progressive increase in [H-3]thymidine. incorporation. These findings indicate that the cells before autologous implantation maintain their differentiated phenotype of mature chondrocytes, and proliferate greatly. This analysis is an essential step preceding wider use of this treatment in humans. However, other biological aspects of the autologous, chondrocyte graft remain to be elucidated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 16:48:03