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Titolo:
Flow cytometric analysis of mitogen-induced activation of rainbow trout (Oncorhynchus mykiss) peripheral blood leucocytes
Autore:
Scharsack, JP; Steinhagen, D; Leibold, W; Rabe, U; Korting, W; Schuberth, HJ;
Indirizzi:
Hannover Sch Vet Med, Fish Dis Res Unit, D-30559 Hannover, Germany Hannover Sch Vet Med Hannover Germany D-30559 D-30559 Hannover, Germany Hannover Sch Vet Med, Immunol Unit, D-30173 Hannover, Germany Hannover SchVet Med Hannover Germany D-30173 D-30173 Hannover, Germany
Titolo Testata:
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARYPUBLIC HEALTH
fascicolo: 5, volume: 48, anno: 2001,
pagine: 331 - 339
SICI:
0931-1793(200106)48:5<331:FCAOMA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARP CYPRINUS-CARPIO; SALMO-GAIRDNERI LEUKOCYTES; CELLULAR-REQUIREMENTS; LYMPHOCYTES; HETEROGENEITY; MACROPHAGES; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Scharsack, JP Hannover Sch Vet Med, Fish Dis Res Unit, Buenteweg 17, D-30559 Hannover, Germany Hannover Sch Vet Med Buenteweg 17 Hannover Germany D-30559
Citazione:
J.P. Scharsack et al., "Flow cytometric analysis of mitogen-induced activation of rainbow trout (Oncorhynchus mykiss) peripheral blood leucocytes", J VET MED B, 48(5), 2001, pp. 331-339

Abstract

Proliferation of rainbow trout peripheral blood leucocytes in vitro is usually assessed by measuring incorporated tritiated thymidine. In this reportwe monitored the in vitro proliferative response to the mitogen Concanavalin A (Con A) by means of flow cytometry (FCM) and H-3-thymidine incorporation. When analysed by FCM, blood leucocytes displayed two main cell populations with distinct forward and side scatter (FSC/SSC) characteristics: lymphocytes with low FSC/SSC values and non-lymphoid leucocytes (NLL) with increased FSC/SSC values. The nature of these cell types were confirmed by microscopy. Interestingly, the FSC/SSC pattern of lymphocytes remained unchangedafter in vitro stimulation with Con A, whereas cells from the NLL population showed a marked shift towards increased FSC values. In stimulated cultures, the increase of FSC values of the NLL population significantly correlated with contemporarily measured H-3-thymidine incorporation (r = 0.7, P < 0.001). The mitogenic response of blood leucocytes originating from different individual fish varied over wide ranges. It was found to be related to the numbers of NLL present in the leucocyte sample. The present results show that qualitative and quantitative FCM analysis of morphological parameters (FSC/SSC) of blood leucocytes makes it possible to discriminate between leucocyte populations of the rainbow trout and to monitor cell proliferation experiments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 20:50:34