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Titolo:
Isotype-specific antibody responses of cattle to Salmonella Dublin lipopolysaccharide and porin following Salmonella Dublin vaccination and acute andchronic infection
Autore:
House, JK; Smith, BR; OConnell, K; VanMetre, DC;
Indirizzi:
Univ Calif Davis, Dept Med & Epidemiol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 Med & Epidemiol, Davis, CA 95616 USA Schering Plough Anim Hlth, Wellington, New Zealand Schering Plough Anim Hlth Wellington New Zealand ellington, New Zealand Colorado State Univ, Vet Teaching Hosp, Ft Collins, CO 80523 USA Colorado State Univ Ft Collins CO USA 80523 osp, Ft Collins, CO 80523 USA
Titolo Testata:
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
fascicolo: 3, volume: 13, anno: 2001,
pagine: 213 - 218
SICI:
1040-6387(200105)13:3<213:IAROCT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; CALIFORNIA DAIRIES; S-TYPHIMURIUM; MAMMARY-GLAND; PREVALENCE; UDDER; COWS; MILK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: House, JK Univ Calif Davis, Dept Med & Epidemiol, Davis, CA 95616 USA UnivCalif Davis Davis CA USA 95616 demiol, Davis, CA 95616 USA
Citazione:
J.K. House et al., "Isotype-specific antibody responses of cattle to Salmonella Dublin lipopolysaccharide and porin following Salmonella Dublin vaccination and acute andchronic infection", J VET D INV, 13(3), 2001, pp. 213-218

Abstract

Stimulation of different T-cell subsets during antigen presentation influences the antibody isotype response to an antigen. Salmonella infection and Salmonella bacterin vaccination are likely to stimulate different T-cell subtypes. The objective of this study was to determine whether there are differences in the isotype response of cattle to Salmonella antigens following Salmonella infection and Salmonella bacterin vaccination. Sera from Salmonella bacterin-vaccinated, experimentally infected, and chronically infected (carrier) adult cattle collected during previous studies was used to evaluate the IgG(1), IgG(2), and IgM isotype responses of cows to Salmonella serotype Dublin lipopolysaccharide (LPS) and porin. Following vaccination and experimental oral infection, IgG(1) titers to LPS and porin rose more quickly and persisted longer than did IgG(2) titers. In contrast to Salmonella infection, bacterin vaccination stimulated a weak response to Salmonella porin. Salmonella infection also induced a higher IgG(2):IgG(1) titer ratio to LPS than did bacterin vaccination. Chronic Salmonella infection induced thehighest LPS and porin IgG(2):IgG(1) titer ratios and the highest correlation between LPS and porin titers. Response operating characteristic curves for each isotype-specific enzyme-linked immunosorbent assay (ELISA) were determined to evaluate the effect of isotype on the sensitivity and specificity of Salmonella ELISA serology for distinguishing sera of Salmonella carriers from those of vaccinated and acutely infected cows. IgG(2) titers to LPSand porin provide a more specific indicator of chronic Salmonella infection status than do IgG(1) titers to the same antigens with little to no loss in sensitivity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:16:58