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Titolo:
Evaluation and use of a nested polymerase chain reaction assay in cats experimentally infected with Bartonella henselae genotype I and Bartonella henselae genotype II
Autore:
Roy, AF; Corstvet, RE; Tapp, RA; OReilly, KL; Cox, HU;
Indirizzi:
Louisiana State Univ, Dept Microbiol & Parasitol, Baton Rouge, LA 70803 USA Louisiana State Univ Baton Rouge LA USA 70803 , Baton Rouge, LA 70803 USA Louisiana State Univ, Louisiana Vet Med Diagnost Lab, Sch Vet Med, Baton Rouge, LA 70803 USA Louisiana State Univ Baton Rouge LA USA 70803 , Baton Rouge, LA 70803 USA Louisiana State Univ, Inst Environm Studies, Baton Rouge, LA 70803 USA Louisiana State Univ Baton Rouge LA USA 70803 , Baton Rouge, LA 70803 USA Louisiana State Univ, Inst Mutagenesis, Baton Rouge, LA 70803 USA Louisiana State Univ Baton Rouge LA USA 70803 , Baton Rouge, LA 70803 USA
Titolo Testata:
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
fascicolo: 4, volume: 13, anno: 2001,
pagine: 312 - 322
SICI:
1040-6387(200107)13:4<312:EAUOAN>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCRATCH DISEASE; ROCHALIMAEA-HENSELAE; CLARRIDGEIAE INFECTION; FORMERLY ROCHALIMAEA; DOMESTIC CATS; PET CATS; SP-NOV; BACTEREMIA; PREVALENCE; STRAINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Roy, AF Louisiana State Univ, Dept Microbiol & Parasitol, Baton Rouge, LA 70803 USA Louisiana State Univ Baton Rouge LA USA 70803 Rouge, LA 70803 USA
Citazione:
A.F. Roy et al., "Evaluation and use of a nested polymerase chain reaction assay in cats experimentally infected with Bartonella henselae genotype I and Bartonella henselae genotype II", J VET D INV, 13(4), 2001, pp. 312-322

Abstract

Cats have been shown to be infected with Bartonella henselae genotype I, B. henselae genotype II, and B. clarridgeiae. Feline bartonellosis infections and the strains involved in these infections are important in both veterinary and human medicine. Nucleic acid amplification methods such as polymerase chain reaction (PCR) are being used in both research and diagnostics astools for understanding many infectious diseases. Bartonella bacteremia incats is detected by blood culture; however, because of the limitations of culture (delayed turnaround time and sensitivity limits), PCR may be a moreefficient method for identifying infected cats. Three distinct PCR assays that could differentiate among B. henselae genotype I, B. henselae genotypeII, and B. clarridgeiae were developed and used to detect as few as 3.2 organisms. Fourteen cats experimentally infected with B. henselae genotype I and B. henselae genotype II were followed by bacterial culture and PCR through the course of infection, including periods of primary and relapsing bacteremia. The PCR assay was positive in I I of the 14 cats for periods of 1-9 weeks after culture became negative. Of the 223 blood specimens that wereculture negative, the PCR assay was positive in 38 (17%) of the specimens. Two of the 14 cats developed relapsing bacteremia. The 2 B. henselae genotypes were amplified in the cats and the bacteremic phase of these infections as determined by PCR lasted for a longer period than previously determined by culture. Using laboratory assays such as PCR to understand the strainsinvolved in feline bartonellosis and the course of the infection is important in the understanding of these zoonotic agents.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 15:58:36