Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Early detection of maedi-visna (ovine progressive pneumonia) virus seroconversion in field sheep samples
Autore:
Varea, R; Monleon, E; Pacheco, C; Lujan, L; Bolea, R; Vargas, MA; Van Eynde, G; Saman, E; Dickson, L; Harkiss, G; Amorena, B; Badiola, JJ;
Indirizzi:
Univ Zaragoza, Dept Anim Pathol, Fac Vet, E-50013 Zaragoza, Spain Univ Zaragoza Zaragoza Spain E-50013 l, Fac Vet, E-50013 Zaragoza, Spain CSIC, Dept Anim Hlth, Agr Res Serv SIA DGA, Zaragoza 50018, Spain CSIC Zaragoza Spain 50018 h, Agr Res Serv SIA DGA, Zaragoza 50018, Spain Innogenet NV, B-9052 Ghent, Belgium Innogenet NV Ghent Belgium B-9052Innogenet NV, B-9052 Ghent, Belgium Univ Edinburgh, Dept Vet Pathol, Easter Bush Vet Ctr, Edinburgh EH25 9RG, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH25 9RGidlothian, Scotland
Titolo Testata:
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
fascicolo: 4, volume: 13, anno: 2001,
pagine: 301 - 307
SICI:
1040-6387(200107)13:4<301:EDOM(P>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; RECOMBINANT PROTEIN ELISA; POLYMERASE-CHAIN-REACTION; SMALL RUMINANTS; LENTIVIRUS INFECTIONS; IMMUNODIFFUSION TEST; GEL IMMUNODIFFUSION; ANTIBODY DETECTION; IMMUNOASSAY; DIAGNOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Varea, R Univ Zaragoza, Dept Anim Pathol, Fac Vet, Miguel Servet 177, E-50013 Zaragoza, Spain Univ Zaragoza Miguel Servet 177 Zaragoza Spain E-50013za, Spain
Citazione:
R. Varea et al., "Early detection of maedi-visna (ovine progressive pneumonia) virus seroconversion in field sheep samples", J VET D INV, 13(4), 2001, pp. 301-307

Abstract

The aim of this work was to investigate whether an enzyme-linked immunosorbent assay (ELISA) was useful for early detection of maedi-visna virus (MVV) infection in sheep under field conditions. An ELISA based on p25 recombinant protein and a gp46 synthetic peptide was used. Sequentially obtained serum samples (n = 1,941) were studied for 4 years. ELISA results were compared with those of the agar gel immunodiffusion (AGID) test, and results of both tests were compared with a reference result established using consensusscores for at least 2 of 3 serologic techniques (AGID, ELISA, and western blotting, which was used to resolve result discrepancies between the other 2 techniques). A total of 247 discrepancies were observed between ELISA andAGID. Of these, 131 were due to an earlier detection of 120 sera by the ELISA and 11 sera by AGID. The remaining discrepancies (116) were due to the presence of false reactions in both tests. Fewer false-negative results were found by ELISA than with AGID (6 vs. 69 sera, respectively), whereas the number of false-positive results was virtually the same for ELISA and AGID (21 vs. 20, respectively). In relation to the reference result, ELISA sensitivity and specificity were 97.8% and 98.2%, respectively, whereas values for AGID were 76.3% and 98.3%, respectively. The agreement between ELISA andthe reference result was higher than that between AGID and the reference result (kappa value: 0.96 and 0.77, respectively). A variation in the ELISA signal (based on optical density) was observed during the study period, suggesting different antibody levels throughout the animal's life. The ELISA was useful for detecting MVV-infected sheep in field conditions and has potential for use in control and eradication programs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:32:21