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Titolo:
Real space refinement of acto-myosin structures from sectioned muscle
Autore:
Chen, LF; Blanc, E; Chapman, MS; Taylor, KA;
Indirizzi:
Florida State Univ, Inst Mol Biophys, Tallahassee, FL 32306 USA Florida State Univ Tallahassee FL USA 32306 ys, Tallahassee, FL 32306 USA
Titolo Testata:
JOURNAL OF STRUCTURAL BIOLOGY
fascicolo: 2-3, volume: 133, anno: 2001,
pagine: 221 - 232
SICI:
1047-8477(200102/03)133:2-3<221:RSROAS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSECT FLIGHT-MUSCLE; 3-DIMENSIONAL IMAGE-RECONSTRUCTION; ELECTRON-MICROSCOPY; MACROMOLECULAR STRUCTURES; MOLECULAR-DYNAMICS; LOW-RESOLUTION; ADP RELEASE; COMPLEX; RIGOR; CONTRACTION;
Keywords:
electron microscopy; tomography; insect flight muscle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Taylor, KA Florida State Univ, Inst Mol Biophys, Tallahassee, FL 32306 USAFlorida State Univ Tallahassee FL USA 32306 ssee, FL 32306 USA
Citazione:
L.F. Chen et al., "Real space refinement of acto-myosin structures from sectioned muscle", J STRUCT B, 133(2-3), 2001, pp. 221-232

Abstract

We have adapted a real space refinement protocol originally developed for high-resolution crystallographic analysis for use in fitting atomic models of actin filaments and myosin subfragment 1 (S1) to 3-D images of thin-sectioned, plastic-embedded whole muscle. The rationale for this effort is to obtain a refinement protocol that will optimize the fit of the model to the density obtained by electron microscopy and correct for poor geometry introduced during the manual fitting of a high-resolution atomic model into a lower resolution 3-D image. The starting atomic model consisted of a rigor acto-Sl model obtained by X-ray crystallography and helical reconstruction ofelectron micrographs. This model was rebuilt to fit 3-D images of rigor insect flight muscle at a resolution of 7 nm obtained by electron tomography and image averaging. Our highly constrained real space refinement resulted in modest improvements in the agreement of model and reconstruction but reduced the number of conflicting atomic contacts by 70% without loss of fit to the 3-D density. The methodology seems to be well suited to the derivation of stereochemically reasonable atomic models that are consistent with experimentally determined 3-D reconstructions computed from electron micrographs. (C) 2000 Academic Press.

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Documento generato il 29/01/20 alle ore 19:21:34