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Titolo:
Classification and reconstruction of a heterogeneous set of electron microscopic images: A case study of GroEL-substrate complexes
Autore:
Falke, S; Fisher, MT; Gogol, EP;
Indirizzi:
Univ Missouri, Sch Biol Sci, Kansas City, MO 64110 USA Univ Missouri Kansas City MO USA 64110 iol Sci, Kansas City, MO 64110 USA Univ Kansas, Med Ctr, Dept Biochem & Mol Biol, Kansas City, KS 66160 USA Univ Kansas Kansas City KS USA 66160 Mol Biol, Kansas City, KS 66160 USA
Titolo Testata:
JOURNAL OF STRUCTURAL BIOLOGY
fascicolo: 2-3, volume: 133, anno: 2001,
pagine: 203 - 213
SICI:
1047-8477(200102/03)133:2-3<203:CAROAH>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRYOELECTRON MICROSCOPY; 3-DIMENSIONAL RECONSTRUCTION; DETERMINING ORIENTATIONS; GLUTAMINE-SYNTHETASE; CRYSTAL-STRUCTURE; CHAPERONIN; RESOLUTION; PARTICLES; PROTEIN; MODEL;
Keywords:
chaperonin; electron microscopy; GroEL; image reconstruction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Gogol, EP Univ Missouri, Sch Biol Sci, Kansas City, MO 64110 USA Univ Missouri Kansas City MO USA 64110 ansas City, MO 64110 USA
Citazione:
S. Falke et al., "Classification and reconstruction of a heterogeneous set of electron microscopic images: A case study of GroEL-substrate complexes", J STRUCT B, 133(2-3), 2001, pp. 203-213

Abstract

Image analysis methods were used to separate images of a large macromolecular complex, the chaperonin GroEL, in a preparation in which it is partially liganded to a nonnative protein substrate, glutamine synthetase. The relatively small difference (similar to6%) in size between the chaperonin in its free and complexed forms, and the absence of gross changes in overall conformation, made separation of the two types of particles challenging. Different approaches were evaluated and used for alignment and classification ofimages, both in two common projections and in three dimensions, yielding 2D averages and a 3D reconstruction. The results of 3D analysis describe theconformational changes effected by binding of this particular protein substrate and demonstrate the utility of 2D analysis as an indicator of structural change in this system. (C) 2001 Academic Press.

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Documento generato il 19/01/20 alle ore 09:20:01