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Titolo:
An ab initio algorithm for low-resolution 3-D reconstructions from cryoelectron microscopy images
Autore:
Yin, ZY; Zheng, YL; Doerschuk, PC;
Indirizzi:
Purdue Univ, Sch Elect & Comp Engn, W Lafayette, IN 47907 USA Purdue UnivW Lafayette IN USA 47907 Comp Engn, W Lafayette, IN 47907 USA
Titolo Testata:
JOURNAL OF STRUCTURAL BIOLOGY
fascicolo: 2-3, volume: 133, anno: 2001,
pagine: 132 - 142
SICI:
1047-8477(200102/03)133:2-3<132:AAIAFL>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
3-DIMENSIONAL RECONSTRUCTION; ELECTRON-MICROGRAPHS; PARTICLES;
Keywords:
cryoelectron microscopy reconstruction; virus structure; expectation maximization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Doerschuk, PC Purdue Univ, Sch Elect & Comp Engn, 1285 Elect Engn Bldg, W Lafayette, IN 47907 USA Purdue Univ 1285 Elect Engn Bldg W Lafayette IN USA47907 SA
Citazione:
Z.Y. Yin et al., "An ab initio algorithm for low-resolution 3-D reconstructions from cryoelectron microscopy images", J STRUCT B, 133(2-3), 2001, pp. 132-142

Abstract

A statistical method for determining low-resolution 3-D reconstructions ofvirus particles from cryoelectron microscope images by an ab initio algorithm is described. The method begins with a novel linear reconstruction method that generates a spherically symmetric reconstruction, which is followedby a nonlinear reconstruction method implementing an expectation-maximization procedure using the spherically symmetric reconstruction as an initial condition and resulting in a reconstruction with icosahedral symmetry. Am important characteristic of the complete method is that very little need be known about the particle before the reconstruction is computed, in particular, only the type of symmetry and inner and outer radii. The method is demonstrated on synthetic cowpea mosaic virus data, and its robustness to 5% errors in the contrast transfer function, 5% errors in the location of the center of the particles in the images, and 5% distortion in the 3-D structurefrom which the images are derived is demonstrated numerically. (C) 2001 Academic Press.

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Documento generato il 09/04/20 alle ore 00:27:27