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Titolo:
GABP FACTORS BIND TO A DISTAL INTERLEUKIN-2 (IL-2) ENHANCER AND CONTRIBUTE TO C-RAF-MEDIATED INCREASE IN IL-2 INDUCTION
Autore:
AVOTS A; HOFFMEYER A; FLORY E; CIMANIS A; RAPP UR; SERFLING E;
Indirizzi:
UNIV WURZBURG,INST PATHOL,DEPT MOL PATHOL,JOSEF SCHNEIDER STR 2 D-97080 WURZBURG GERMANY UNIV WURZBURG,INST PATHOL,DEPT MOL PATHOL D-97080 WURZBURG GERMANY UNIV WURZBURG,INSST MED RADIAT RES & CELL BIOL D-97080 WURZBURG GERMANY LATVIAN STATE UNIV,BIOMED RES & STUDY CTR LV-1067 RIGA LATVIA
Titolo Testata:
Molecular and cellular biology
fascicolo: 8, volume: 17, anno: 1997,
pagine: 4381 - 4389
SICI:
0270-7306(1997)17:8<4381:GFBTAD>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL ANTIGEN RECEPTOR; T-CELLS; TRANSCRIPTION FACTORS; SIGNAL-TRANSDUCTION; GENE-EXPRESSION; PROTEIN; ACTIVATION; DNA; PROMOTER; ELF-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
A. Avots et al., "GABP FACTORS BIND TO A DISTAL INTERLEUKIN-2 (IL-2) ENHANCER AND CONTRIBUTE TO C-RAF-MEDIATED INCREASE IN IL-2 INDUCTION", Molecular and cellular biology, 17(8), 1997, pp. 4381-4389

Abstract

Triggering of the T-cell receptor-CD3 complex activates two major signal cascades in T lymphocytes, (i) Ca2+-dependent signal cascades and (ii) protein kinase cascades, Both signal cascades contribute to the induction of the interleukin 2 (IL-2) gene during T-cell activation, Prominent protein kinase cascades are those that activate mitogen-activated protein (MAP) kinases, We show here that c-Raf, which is at the helm of the classic MAP-Erk cascade, contributes to IL-2 induction through a distal enhancer element spanning the nucleotides from positions -502 to -413 in front of the transcriptional start site of the IL-2 gene, Induction of this distal IL-2 enhancer differs from induction of the proximal IL-2 promoter-enhancer, since it is induced by phorbol esters alone and independent from Ca2+ signals, In DNA-protein binding studies, we detected the binding of transcription factors GABP alpha and -beta to a dyad symmetry element (DSE) of the distal enhancer, which is formed by palindromic binding sites of Ets-like factors, Introduction of point mutations suppressing GABP binding to the DSE interfered with the induction of the distal enhancer and the entire IL-2 promoter-enhancer, while overexpression of both GABP factors enhanced the IL-2 promoter-enhancer induction, Overexpression of BXB, a constitutive active version of c-Raf, and of further members of the Ras-Raf-Erk signal cascade exerted an increase of GABP-mediated promoter-enhancer induction, In conjunction with previously published data on c-Raf-induced phosphorylation of GABP factors (E. Flory, A. Hoffmeyer, U. Smola, U. R. Rapp, and J. T. Bruder, J. Virol, 70:2260-2268, 1996), these results indicate a contribution of GABP factors to the Raf-mediated enhancementof IL-2 induction during T-cell activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 20:47:59