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Titolo:
Thymic epithelial cell line expresses transcripts encoding alpha-3, alpha-5 and beta-4 subunits of acetylcholine receptors, responds to cholinergic agents and expresses choline acetyl transferase. An in vitro system to investigate thymic cholinergic mechanisms
Autore:
Mihovilovic, M; Butterworth-Robinette, J;
Indirizzi:
Duke Univ, Dept Med, Div Neurol, Durham, NC 27710 USA Duke Univ Durham NCUSA 27710 Dept Med, Div Neurol, Durham, NC 27710 USA
Titolo Testata:
JOURNAL OF NEUROIMMUNOLOGY
fascicolo: 1-2, volume: 117, anno: 2001,
pagine: 58 - 67
SICI:
0165-5728(20010702)117:1-2<58:TECLET>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NICOTINIC RECEPTORS; PREFERENTIAL TRANSCRIPTION; HUMAN KERATINOCYTES; MYASTHENIA-GRAVIS; THYMOCYTES; ACETYLTRANSFERASE; DIFFERENTIATION; LYMPHOCYTES; ADHESION; GENES;
Keywords:
acetylcholine receptors; thymus; thymocytes; thymic epithelia; neuroimmune; myasthenia gravis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Mihovilovic, M Duke Univ, Dept Med, Div Neurol, DUMC Box 2900, Durham, NC 27710 USA Duke Univ DUMC Box 2900 Durham NC USA 27710 m, NC 27710 USA
Citazione:
M. Mihovilovic e J. Butterworth-Robinette, "Thymic epithelial cell line expresses transcripts encoding alpha-3, alpha-5 and beta-4 subunits of acetylcholine receptors, responds to cholinergic agents and expresses choline acetyl transferase. An in vitro system to investigate thymic cholinergic mechanisms", J NEUROIMM, 117(1-2), 2001, pp. 58-67

Abstract

Transcriptional and immunocytological characterization of thymic epithelial (TE) cell line TE750 shows that these cells, like primary TE cell cultures, transcribe alpha -3, alpha -5 and beta -4 acetylcholine receptor (AcChR)subunit genes while expressing cortical, medullary and epithelial differentiation thymic markers. Incubation of TE750 cells with nicotine decreases cell adherence and growth as measured through direct cytological observationand nucleic acid quantification, respectively. Physostigmine, a traditional cholinesterase inhibitor that also activates nicotinic AcChRs, reproducesthe effects of nicotine. Strengthening the hypothesis that cholinergic receptors mediate the effects of physostigmine, acetylcholinesterase (AcChase)activity is not detected in TE750 cells. Also, like thymocytes, TE750 cells express choline acetyltransferase (ChAT), indicating that the natural transmitter AcCh can be produced locally within the thymic parenchyma. Taken together these findings indicate that TE750 cells in culture represent a suitable in vitro system for the analysis of cholinergic mechanisms operational in the thymic epithelium. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 10:01:09