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Titolo:
Immunogenic and isotype-specific responses to Russian and US cold-adapted influenza A vaccine donor strains A/Leningrad/134/17/57, A/Leningrad/134/47/57, and A/Ann Arbor/6/60 (H2N2) in mice
Autore:
Wareing, MD; Watson, JM; Brooks, MJ; Tannock, GA;
Indirizzi:
RMIT Univ, Dept Biotechnol & Environm Biol, Bundoora, Vic, Australia RMIT Univ Bundoora Vic Australia Environm Biol, Bundoora, Vic, Australia
Titolo Testata:
JOURNAL OF MEDICAL VIROLOGY
fascicolo: 1, volume: 65, anno: 2001,
pagine: 171 - 177
SICI:
0146-6615(200109)65:1<171:IAIRTR>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
FORMING CELL RESPONSE; VIRUS HEMAGGLUTININ; SUBCLASS RESPONSES; SEQUENCE CHANGES; WILD-TYPE; A VIRUS; ANTIBODY; LIVE; INFECTION; CHALLENGE;
Keywords:
immunogenicity; cold-adapted; influenza; vaccine; donor strain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Tannock, GA RMIT Univ, Dept Biotechnol & Environm Biol, POB 71, Bundoora, Vic, Australia RMIT Univ POB 71 Bundoora Vic Australia doora, Vic, Australia
Citazione:
M.D. Wareing et al., "Immunogenic and isotype-specific responses to Russian and US cold-adapted influenza A vaccine donor strains A/Leningrad/134/17/57, A/Leningrad/134/47/57, and A/Ann Arbor/6/60 (H2N2) in mice", J MED VIROL, 65(1), 2001, pp. 171-177

Abstract

The immunogenicity of the Russian cold-adapted (ca) donor stains, A/Leningrad (Len)/134/17/57 and A/Leningrad/134/47/57, and the US strain A/Ann Arbor (AA)/6/60-ca, were compared in BALB/c mice with their respective wild-type parental viruses. Each ca donor strain was less immunogenic than. its wild-type parent. The vaccinating dose, when administered twice, which prevented multiplication of a standard challenge of parental wild-type virus in 50% of mice (the 50% protective dose or PD50), was shown for A/Len/134/17/57-ca, A/Len/134/47/57-ca, and A/AA/6/60-ca to be 10(3.77), 10(4.32), and 10(4.70), respectively. These findings were extended by measuring the number ofantibody secreting cells induced in the lungs and mediastinal lymph nodes of mice infected with the same ca donors using an ELISPOT assay. When each donor strain was administered twice at a dose of 100 PD50 over a 3-week interval, the overall immunoglobulin isotype antibody secreting cell profiles were shown to be similar. However, A/Len/134/17/57-ca and A/Len/134/47/57-ca induced significantly higher total immunoglobulin responses in the lungs than A/AA/6/60-ca (P <0.05). A/Len/134/17/57-ca also induced a significantly greater IgA response in the lungs than A/AA/6/60-ca (P <0.05). These results suggest that A/Len/134/17/57-ca is a superior immunogen to A/Len/134/47/57-ca which in turn is more immunogenic than A/AA/6/60-ca. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 03:30:47