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Titolo:
Kaposi's sarcoma-associated herpesvirus serology in Europe and Uganda: Multicentre study with multiple and novel assays
Autore:
Schatz, O; Monini, P; Bugarini, R; Neipel, F; Schulz, TF; Andreoni, M; Erb, P; Eggers, M; Haas, J; Butto, S; Lukwiya, M; Bogner, JR; Yaguboglu, S; Sheldon, J; Sarmati, L; Goebel, FD; Hintermaier, R; Enders, G; Regamey, N; Wernli, M; Sturzl, M; Rezza, G; Ensoli, B;
Indirizzi:
Univ Munich, Med Poliklin, Infekt Lab, D-8000 Munich, Germany Univ MunichMunich Germany D-8000 in, Infekt Lab, D-8000 Munich, Germany Ist Super Sanita, Virol Lab, I-00161 Rome, Italy Ist Super Sanita Rome Italy I-00161 nita, Virol Lab, I-00161 Rome, Italy Ist Super Sanita, Epidemiol & Biostat Lab, I-00161 Rome, Italy Ist Super Sanita Rome Italy I-00161 l & Biostat Lab, I-00161 Rome, Italy Univ Erlangen Nurnberg, Inst Clin & Med Virol, Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany & Med Virol, Erlangen, Germany Univ Liverpool, Dept Med Microbiol, Liverpool L69 3BX, Merseyside, EnglandUniv Liverpool Liverpool Merseyside England L69 3BX , Merseyside, England Univ Roma Tor Vergata, Dept Infect Dis, I-00173 Rome, Italy Univ Roma Tor Vergata Rome Italy I-00173 Infect Dis, I-00173 Rome, Italy Univ Basel, Inst Med Microbiol, Basel, Switzerland Univ Basel Basel Switzerland el, Inst Med Microbiol, Basel, Switzerland Inst Virol Infectiol & Epidemiol, Med Diagnost Gemeinschaftslab, Stuttgart, Germany Inst Virol Infectiol & Epidemiol Stuttgart Germany , Stuttgart, Germany Max Von Pettenkofer Inst, Genzentrum, D-8000 Munich, Germany Max Von Pettenkofer Inst Munich Germany D-8000 m, D-8000 Munich, Germany St Marys Hosp Lacor, Gulu, Uganda St Marys Hosp Lacor Gulu UgandaSt Marys Hosp Lacor, Gulu, Uganda GSF, Natl Res Ctr Environm & Hlth, Inst Mol Virol, Neuherberg, Germany GSF Neuherberg Germany ronm & Hlth, Inst Mol Virol, Neuherberg, Germany
Titolo Testata:
JOURNAL OF MEDICAL VIROLOGY
fascicolo: 1, volume: 65, anno: 2001,
pagine: 123 - 132
SICI:
0146-6615(200109)65:1<123:KSHSIE>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD MONONUCLEAR-CELLS; TO-CHILD TRANSMISSION; PERIPHERAL-BLOOD; DNA-SEQUENCES; HUMAN-HERPESVIRUS-8 INFECTION; TRANSPLANT RECIPIENTS; CASTLEMANS-DISEASE; SPINDLE CELLS; ANTIBODIES; SEROPREVALENCE;
Keywords:
organ transplantation; risk management; serodiagnosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Schatz, O DIAVIR Ltd, Steinbergstr 30, D-85250 Altomuenster, Germany DIAVIR Ltd Steinbergstr 30 Altomuenster Germany D-85250 Germany
Citazione:
O. Schatz et al., "Kaposi's sarcoma-associated herpesvirus serology in Europe and Uganda: Multicentre study with multiple and novel assays", J MED VIROL, 65(1), 2001, pp. 123-132

Abstract

A multicentre study was undertaken to define novel assays with increased inter-assay concordance, sensitivity, specificity and predictive value for serological diagnosis of human herpesvirus type 8 (HHV-8) infection, A totalof 562 sera from European and Ugandan human immunodeficiency virus (HIV)-infected or uninfected individuals with or without Kaposi's sarcoma (KS) andblood donors were examined under code by 18 different assays in seven European laboratories. Sera from KS patients and all non-KS sera found positiveby at least 70%,80%, or 90% of the assays were considered "true positive. "The validity of the assays was then evaluated by univariate logistic regression analysis. Two immunofluorescence assays (IFA) for detection of antibodies against HHV-8 lytic (Rlyt) or latent (LLANA) antigens and two enzyme-linked-immunosorbent assays (ELISA) (M2, EK8.1) for detection of antibodies against HHV-8 structural proteins were found to be highly concordant, specific, and sensitive, with odds ratios that indicated a high predictive value. When used together, the two IFA (Rlyt-LLANA) showed the best combination of sensitivity (89.1%) and specificity (94.9%). The performance of these assays indicate that they may be used for the clinical management of individuals at risk of developing HHV-8 associated tumours such as allograft recipients. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 13/07/20 alle ore 06:45:09