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Titolo:
Human monocyte-derived dendritic cells produce bioactive gelatinase B: Inhibition by IFN-ss
Autore:
Bartholome, EJ; Van Aelst, I; Koyen, E; Kiss, R; Willems, F; Goldman, M; Opdenakker, G;
Indirizzi:
Free Univ Brussels, Hop Erasme, Dept Immunol, B-1070 Brussels, Belgium Free Univ Brussels Brussels Belgium B-1070 nol, B-1070 Brussels, Belgium Free Univ Brussels, Ctr Rech Interuniv Vaccinol, B-1070 Brussels, Belgium Free Univ Brussels Brussels Belgium B-1070 nol, B-1070 Brussels, Belgium Free Univ Brussels, Histol Lab, B-1070 Brussels, Belgium Free Univ Brussels Brussels Belgium B-1070 Lab, B-1070 Brussels, Belgium Catholic Univ Louvain, Rega Inst Med Res, Lab Mol Immunol, B-3000 Louvain,Belgium Catholic Univ Louvain Louvain Belgium B-3000 nol, B-3000 Louvain,Belgium
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 7, volume: 21, anno: 2001,
pagine: 495 - 501
SICI:
1079-9907(200107)21:7<495:HMDCPB>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT INTERFERON-BETA; MULTIPLE-SCLEROSIS; MATRIX METALLOPROTEINASES; IN-VITRO; IDENTIFICATION; EXPRESSION; MIGRATION; MATRIX-METALLOPROTEINASE-9; CARCINOMA; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Bartholome, EJ Free Univ Brussels, Hop Erasme, Dept Immunol, 808 Route Lennik, B-1070 Brussels, Belgium Free Univ Brussels 808 Route Lennik BrusselsBelgium B-1070
Citazione:
E.J. Bartholome et al., "Human monocyte-derived dendritic cells produce bioactive gelatinase B: Inhibition by IFN-ss", J INTERF CY, 21(7), 2001, pp. 495-501

Abstract

We studied the secretion of gelatinase B by dendritic cells (DC) generatedby culturing human peripheral blood monocytes in granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). First, we found the intracellular expression of gelatinase B on sections of fixed DC pellets. Zymography analysis of the supernatants of DC cultured for 72 h demonstrated the presence of gelatinase B. To determine if DC produce net enzymaticactivity, bioactive gelatinase, a novel sensitive fluorescent-activated substrate conversion (FASC) assay was used to complement the zymography data. Culture media of unstimulated DC demonstrated reproducible net gelatinolytic activity. Tumor necrosis factor-alpha (TNF-alpha) IL-1 beta but not lipopolysaccharide (LPS) stimulation caused a significant increase in gelatinase B production in zymography analysis. Both types of stimulation failed to increase net gelatinase activity in FASC assay. Interestingly, interferon-beta (IFN-beta) significantly diminished both the total zymolytic productionand the net bioactive gelatinase produced by DC in a dose-dependent manner. We conclude that human monocyte-derived DC secrete bioactive gelatinase Band that IFN-beta inhibits this production.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/08/20 alle ore 16:03:16