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Titolo:
Identification of 54 mycobacterial species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene
Autore:
Brunello, F; Ligozzi, M; Cristelli, E; Bonora, S; Tortoli, E; Fontana, R;
Indirizzi:
Univ Verona, Dipartimento Patol, Sez Microbiol, I-37100 Verona, Italy UnivVerona Verona Italy I-37100 l, Sez Microbiol, I-37100 Verona, Italy Azienda Ospedaliera Verona, Microbiol Serv, I-37100 Verona, Italy Azienda Ospedaliera Verona Verona Italy I-37100 v, I-37100 Verona, Italy Univ Turin, Clin Malattie Infett, Turin, Italy Univ Turin Turin ItalyUniv Turin, Clin Malattie Infett, Turin, Italy Osped Careggi Firenze, Microbiol Serv, Florence, Italy Osped Careggi Firenze Florence Italy e, Microbiol Serv, Florence, Italy Ctr Riferimento Nazl Micobatteri, Florence, Italy Ctr Riferimento Nazl Micobatteri Florence Italy atteri, Florence, Italy
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 8, volume: 39, anno: 2001,
pagine: 2799 - 2806
SICI:
0095-1137(200108)39:8<2799:IO5MSB>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAPIDLY GROWING MYCOBACTERIA; POLYMERASE-CHAIN-REACTION; RIBOSOMAL-RNA; CLINICAL SPECIMENS; ENZYME ANALYSIS; LIQUID-MEDIA; ROUTINE USE; DNA; KANSASII; AMPLIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Fontana, R Univ Verona, Dipartimento Patol, Sez Microbiol, Strada Grazie 8, I-37100 Verona, Italy Univ Verona Strada Grazie 8 Verona Italy I-37100 Verona, Italy
Citazione:
F. Brunello et al., "Identification of 54 mycobacterial species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene", J CLIN MICR, 39(8), 2001, pp. 2799-2806

Abstract

A total of 121 reference and clinical strains of both slowly and rapidly growing mycobacteria belonging to 54 species were studied for restriction fragment length polymorphism of a PCR-amplified 439-bp segment of the gene encoding the 65-kDa heat shock protein. Restriction digests were separated by10% polyacrylamide gel electrophoresis (PAGE). By including a size standard in each sample, the restriction fragment profile was calculated using a computer-aided comparison program. An algorithm describing these 54 species (including 22 species not previously described) is proposed. We found that this assay based on 10% PAGE provided a more precise estimate than that based on agarose gel electrophoresis of the real size of restriction fragmentsas deduced from the sequence analysis and allowed identification of mycobacteria whose PCR-restriction fragment length polymorphism analysis patternswere unequivocally identified by fragments shorter than 60 bp.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:31:00