Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Targeting expression of hsp70i to discrete neuronal populations using the Lmo-1 promoter: Assessment of the neuroprotective effects of hsp70i in vivoand in vitro
Autore:
Kelly, S; Bieneman, A; Horsburgh, K; Hughes, D; Sofroniew, MV; McCulloch, J; Uney, JB;
Indirizzi:
Univ Bristol, URCN, Bristol, Avon, England Univ Bristol Bristol Avon England Bristol, URCN, Bristol, Avon, England Univ Bristol, Div Med, MRC, Ctr Synapt Plast, Bristol, Avon, England Univ Bristol Bristol Avon England r Synapt Plast, Bristol, Avon, England Univ Glasgow, Wellcome Surg Inst, Glasgow, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland urg Inst, Glasgow, Lanark, Scotland Univ Calif Los Angeles, Sch Med, Brain Res Inst, Los Angeles, CA 90024 USAUniv Calif Los Angeles Los Angeles CA USA 90024 Los Angeles, CA 90024 USA
Titolo Testata:
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
fascicolo: 8, volume: 21, anno: 2001,
pagine: 972 - 981
SICI:
0271-678X(200108)21:8<972:TEOHTD>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK; MESSENGER-RNA; PROTEIN-SYNTHESIS; CULTURED NEURONS; STRESS; CELLS; APOPTOSIS; DNAJ; RAT; HEAT-SHOCK-PROTEIN-70;
Keywords:
global ischemia cell culture; glutamate; heat shock proteins; Lmo-1 promoter; transient forebrain ischemia; transgenic mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Uney, JB Univ Bristol, URCN, Marlborough St, Bristol, Avon, England Univ Bristol Marlborough St Bristol Avon England , Avon, England
Citazione:
S. Kelly et al., "Targeting expression of hsp70i to discrete neuronal populations using the Lmo-1 promoter: Assessment of the neuroprotective effects of hsp70i in vivoand in vitro", J CEREBR B, 21(8), 2001, pp. 972-981

Abstract

Transgenic technology provides a powerful means of studying gene regulation and specific gene function with complex mammalian systems. In this study,the authors exploited the specific and discrete neuronal expression pattern mediated by promoter I of the Lmo-1 gene to study the neuroprotective effects of the inducible form of heat shock protein 70kD (hsp70i) in primary hippocampal cultures in a mouse model of global cerebral ischemia. Targetingexpression of hsp70i to hippocampal neurons protected these cells significantly from toxic levels of glutamate and oxidative stress (for example, exposure to 10 mu mol/L free iron produced a 26% increase in lactate dehydrogenase release from neurons cultured from wild-type mice, but a 7% increase in neurons cultured from hsp70i transgenic mice). Bilateral carotid occlusion (25 minutes) produced significantly less neuronal damage in the caudate nucleus and posterior thalamus in hsp70i transgenic mice than in wild-type littermates (for example, 21% +/- 9.3% and 12.5% +/- 9.0% neuronal damage inlateral caudate nucleus of wild-type and hsp70i transgenic mice, respectively, P < 0.05). The current study highlights the utility of targeted expression of transgenes of interest in cerebral ischemia and demonstrates that expression of hsp70i alone is sufficient to mediate the protection of primary neurons from denaturing stress and that expression of human hsp70i in vivo plays crucial role in determining the fate of neurons after ischemic challenge.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:44:51