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Titolo:
Molecular and cellular mechanisms of adipose secretion: Comparison of leptin and angiotensinogen
Autore:
Turban, S; Hainault, I; Andre, J; Ferre, P; Quignard-Boulange, A; Guerre-Millo, M;
Indirizzi:
INSERM, U 465, F-75006 Paris, France INSERM Paris France F-75006INSERM, U 465, F-75006 Paris, France
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 4, volume: 82, anno: 2001,
pagine: 666 - 673
SICI:
0730-2312(2001)82:4<666:MACMOA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; PLASMINOGEN-ACTIVATOR INHIBITOR-1; OB GENE-EXPRESSION; 3T3-L1 ADIPOCYTES; RAT ADIPOCYTES; OBESE GENE; GLUCOSE-METABOLISM; INSULIN-RESISTANCE; TARGET GENE; TISSUE;
Keywords:
adipose cell; obesity; brefeldin A; monensin; Golgi apparatus; insulin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Guerre-Millo, M INSERM, U 465, 15 Rue Ecole Med, F-75006 Paris, France INSERM 15 Rue Ecole Med Paris France F-75006 aris, France
Citazione:
S. Turban et al., "Molecular and cellular mechanisms of adipose secretion: Comparison of leptin and angiotensinogen", J CELL BIOC, 82(4), 2001, pp. 666-673

Abstract

Besides their function of lipid storage, the adipose cells secrete a number of proteins of physiopathological importance. To get further insights into this function, which remains poorly characterized, we sought to compare the mechanisms and regulation of secretion of two individual proteins in thesame cells. Leptin and angiotensinogen were chosen and assessed by radioimmunoassay and quantitative immunoblotting, respectively, in primary cultureof epididymal adipose cells from young obese Zucker rats. Leptin was secreted at a steady rate of 4 ng/10(6) cells/h over 2-6 h. Despite secretion, leptin cellular content remained stable at 3 ng/10(6) cells. In contrast, the rate of angiotensinogen secretion decreased regularly from 45 arbitrary units/10(6) cells/h at 2 h, to half this value at 6 h, although cell contentremained constant at 100 arbitrary units/10(6) cells. Inhibition of protein synthesis by cycloheximide depleted the cells from leptin, but not from angiotensinogen for up to 6 h. Insulin increased leptin secretion (+75%) andcell content (+70 %), without affecting angiotensinogen. Secretion of bothproteins was inhibited by Golgi-disturbing agents, brefeldin A and monensin. The presence of brefeldin A led to a specific rise in leptin cell content, an effect inhibited by cycloheximide and enhanced by insulin (+80%). These data show that leptin and angiotensinogen are both secreted through Golgi-dependent pathways and that their respective intracellular pool exhibit distinct turn-over rate and insulin sensitivity. These characteristics mightaccount for the differential response of these adipose proteins to variations in the systemic environment. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 22:19:23