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Titolo:
A comparative study on two GNRA-tetraloop receptors: 11-nt and IC3 motifs
Autore:
Ikawa, Y; Nohmi, K; Atsumi, S; Shiraishi, H; Inoue, T;
Indirizzi:
Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Grad Sch Biostudies, Kyoto 6068502, Japan
Titolo Testata:
JOURNAL OF BIOCHEMISTRY
fascicolo: 2, volume: 130, anno: 2001,
pagine: 251 - 255
SICI:
0021-924X(200108)130:2<251:ACSOTG>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO SELECTION; TRANSFER-RNA GENES; TETRAHYMENA RIBOZYME; TERTIARY STRUCTURE; GAAA TETRALOOP; INTRONS; EVOLUTION; DOMAIN; P5ABC;
Keywords:
evolution; GNRA loop; intron; ribozyme; RNA motif;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Inoue, T Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan Kyoto UnivKyoto Japan 6068502 Biostudies, Kyoto 6068502, Japan
Citazione:
Y. Ikawa et al., "A comparative study on two GNRA-tetraloop receptors: 11-nt and IC3 motifs", J BIOCHEM, 130(2), 2001, pp. 251-255

Abstract

Natural RNAs often contain terminal loops consisting of GNRA (N=A, G, C, U; R=A, G) and their receptors, which bind to the loops via long-range RNA-RNA interactions. Among several known receptors, two characteristic structural elements have been identified that are termed the 11-nt motif (CCUAAG-UAUGG) and IC3 motif (CCCUAAC-GAGGG). These two motifs that share a similar secondary structure have been shown to exhibit distinctively different binding specificities. The 11-nt motif recognizes a GAAA loop with highest specificity among the known receptors, whereas the IC3 motif distinguishes GAAA from other GNRA loops less stringently than any other receptors. To identify the elements in the receptors that determine the binding specificity, a series of chimeric receptors derived from the two motifs were prepared and their properties were examined. We identified characteristic base-pairs and a particular U residue in the receptors as such elements by means of a gel mobility shift assay that evaluates the degree of the tetraloop-receptor interaction. The relationship between the elements and the specificity is discussed together with a model that describes a possible evolutional linkage between the two receptors.

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Documento generato il 25/01/20 alle ore 09:32:12