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Titolo:
Decreased expression of P-glycoprotein in interleukin-1 beta and interleukin-6 treated rat hepatocytes
Autore:
Sukhai, M; Yong, A; Pak, A; Piquette-Miller, M;
Indirizzi:
Univ Toronto, Fac Pharm, Toronto, ON M5S 2S2, Canada Univ Toronto TorontoON Canada M5S 2S2 Pharm, Toronto, ON M5S 2S2, Canada
Titolo Testata:
INFLAMMATION RESEARCH
fascicolo: 7, volume: 50, anno: 2001,
pagine: 362 - 370
SICI:
1023-3830(200107)50:7<362:DEOPII>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIDRUG-RESISTANCE; GENE-EXPRESSION; NORMAL-TISSUES; MESSENGER-RNA; CELL-LINES; LIVER; MDR1B; INDUCTION; CYTOKINES; CULTURES;
Keywords:
cytokines; multidrug resistance; P-glycoprotein; gene regulation; hepatocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Piquette-Miller, M Univ Toronto, Fac Pharm, 19 Russell St, Toronto, ON M5S2S2, Canada Univ Toronto 19 Russell St Toronto ON Canada M5S 2S2 da
Citazione:
M. Sukhai et al., "Decreased expression of P-glycoprotein in interleukin-1 beta and interleukin-6 treated rat hepatocytes", INFLAMM RES, 50(7), 2001, pp. 362-370

Abstract

Objective and Design: As acute inflammation is known to cause a reduction in hepatic P-Glycoprotein (PGP) expression and activity in rats, we tested the hypothesis that the pro-inflammatory cytokines interleukin (IL-)1 beta and IL-6 also mediate reductions in PGRMethods: Hepatocytes were incubated with 0-50 ng/ml of cytokine for 24-72 h. PGP/mdr expression was examined by immunodetection and quantitative RT-PCR analysis and PGP efflux activity was assayed. Results: PGP protein was significantly reduced in cells treated for 3 dayswith IL-1 beta and 24 It with IL-6 (p < 0.05), maximal effects occurring at 5 ng/ml for each cytokine. PGP activity was reduced in both IL-1 beta andIL-6 treated cells (p <0.05). mdr1 mRNA was decreased in cells treated with IL-6, but not IL-1 beta. spgp and mdr2 were not affected. Conclusions: Our data indicate that IL-6 and IL-1 beta have suppressive effects on the expression and activity of PGP in cultured hepatocytes, likelyoccurring through distinct mechanisms. These cytokines may have a potential role in PGP regulation during inflammatory responses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 06:59:03