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Titolo:
Ascorbic acid and N-acetylcysteine improve in vitro the function of lymphocytes from mice with endotoxin-induced oxidative stress
Autore:
De la Fuente, M; Victor, VM;
Indirizzi:
Univ Complutense, Fac Biol, Dept Anim Physiol, E-28040 Madrid, Spain Univ Complutense Madrid Spain E-28040 nim Physiol, E-28040 Madrid, Spain
Titolo Testata:
FREE RADICAL RESEARCH
fascicolo: 1, volume: 35, anno: 2001,
pagine: 73 - 84
SICI:
1071-5762(2001)35:1<73:AAANII>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; TNF-ALPHA PRODUCTION; HUMAN SEPTIC SHOCK; MACROPHAGE FUNCTION; PROTEIN-KINASE; IN-VITRO; ANTIOXIDANTS; LIPOPOLYSACCHARIDE; MODULATION; ACTIVATION;
Keywords:
endotoxic shock; lymphocytes; lipopolysaccharide; antioxidants; oxidative stress;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: De la Fuente, M Univ Complutense, Fac Biol, Dept Anim Physiol, E-28040 Madrid, Spain Univ Complutense Madrid Spain E-28040 8040 Madrid, Spain
Citazione:
M. De la Fuente e V.M. Victor, "Ascorbic acid and N-acetylcysteine improve in vitro the function of lymphocytes from mice with endotoxin-induced oxidative stress", FREE RAD RE, 35(1), 2001, pp. 73-84

Abstract

Oxidative stress associated with reactive oxygen species (ROS) and cytokines produced by immune cells, which is involved in septic shock caused by endotoxin, can be controlled to a certain degree by antioxidants with free radical scavenging action. N-acetylcysteine (NAC) and ascorbic acid (AA) are ROS scavengers that improve the immune response, and modulate macrophage function in mice with endotoxin-caused oxidative stress. Therefore, we have investigated the in vitro effects of these antioxidants on the functions of lymphocytes from BALB/c mice with lethal endotoxic shock caused by intraperitoneal injection of E. coli lipopolysaccharide (LPS) (100mg/kg). Adherenceto tissues and chemotaxis (the earliest two functions of lymphocytes in the immune response), as well as ROS levels and TNF alpha production were determined in the presence or absence of NAC or AA (0.001, 0.01, 0.1, 1 and 2.5 mM) in lymphocytes from peritoneum, axillary nodes, spleen and thymus obtained at several times (2, 4, 12 and 24 hours) after LPS injection. Endotoxic shock decreases the chemotaxis of lymphocytes from all the above localizations and increases their adherence, TNFa and ROS production. These changes in lymphocyte function were counteracted by NAC and AA, bringing these functions to values near those of control animals. Our data suggest that lymphocytes are important targets of endotoxins contributing to oxidative stress by septic shock, and that antioxidants can preserve the function of lymphocytes, preventing the homeostatic disturbances caused by endotoxin.

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Documento generato il 05/07/20 alle ore 10:30:06