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Titolo:
Role of reactive oxygen species generated by NADPH oxidase in the mechanism of activation of K+-Cl--cotransport by N-ethylmaleimide in HepG2 human hepatoma cells
Autore:
Kim, JA; Lee, YS;
Indirizzi:
Kwandong Univ, Coll Med, Dept Physiol, Kangnung 210701, South Korea Kwandong Univ Kangnung South Korea 210701 , Kangnung 210701, South Korea Yeungnam Univ, Coll Pharm, Kyongsan 712749, South Korea Yeungnam Univ Kyongsan South Korea 712749 , Kyongsan 712749, South Korea
Titolo Testata:
FREE RADICAL RESEARCH
fascicolo: 1, volume: 35, anno: 2001,
pagine: 43 - 53
SICI:
1071-5762(2001)35:1<43:ROROSG>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN PHOSPHATASE; KCL COTRANSPORT; FLUORESCENT INDICATORS; POTASSIUM CHANNELS; HUMAN ERYTHROCYTES; HYDROGEN-PEROXIDE; ENDOTHELIAL-CELLS; VOLUME REGULATION; RAT HEPATOCYTES; CHLORIDE;
Keywords:
K+-Cl--cotransport; reactive oxygen species; NADPH oxidase; N-ethylmaleimide; HepG2 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, YS Kwandong Univ, Coll Med, Dept Physiol, Kangnung 210701, South Korea Kwandong Univ Kangnung South Korea 210701 ng 210701, South Korea
Citazione:
J.A. Kim e Y.S. Lee, "Role of reactive oxygen species generated by NADPH oxidase in the mechanism of activation of K+-Cl--cotransport by N-ethylmaleimide in HepG2 human hepatoma cells", FREE RAD RE, 35(1), 2001, pp. 43-53

Abstract

K+-Cl--cotransport (KCC) is ubiquitously present in all cells, and plays an essential role in ion and volume regulation. In this study we investigated the role of reactive oxygen species (ROS) in regulation of KCC in HepG2 human hepatoblastoma cells. N-ethylmaleimide (NEM), a KCC activator, inducedCl--dependent K+ efflux, which was markedly prevented by KCC inhibitors (calyculin-A, genistein and BaCl2), indicating that KCC is activated by NEM in the HepG2 cells. Treatment with NEM also induced a sustained increase in the level of intracellular ROS assessed by 2',7'-dichlorofluorescein flourescence. Antioxidants, N-acetyl cysteine or N,N'-diphenyl-p-phenylenediaminesignificantly inhibited both ROS generation and KCC activation induced by NEM. The NEM-induced ROS production was significantly suppressed by inhibitors of NADPH oxidase (diphenylene iodonium, apocynin and neopterine). Theseinhibitors also significantly inhibited the NEM-induced KCC activation. Taken together, these results suggest that ROS generated by NADPH oxidase maymediate the NEM-induced activation of KCC in human hepatoma cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 21:12:50