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Titolo:
alpha-Tocopherol, but not gamma-tocopherol inhibits 7 beta-hydroxycholesterol-induced apoptosis in human U937 cells
Autore:
Lyons, NM; Woods, JA; OBrien, NM;
Indirizzi:
Natl Univ Ireland Univ Coll Cork, Dept Food Sci Food Technol & Nutr, Cork,Ireland Natl Univ Ireland Univ Coll Cork Cork Ireland hnol & Nutr, Cork,Ireland
Titolo Testata:
FREE RADICAL RESEARCH
fascicolo: 3, volume: 35, anno: 2001,
pagine: 329 - 339
SICI:
1071-5762(2001)35:3<329:ABNGI7>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ENDOTHELIAL-CELLS; A REDUCTASE-ACTIVITY; SMOOTH-MUSCLE CELLS; CHOLESTEROL OXIDATION; 7-KETOCHOLESTEROL-INDUCED APOPTOSIS; OXYSTEROLS; ATHEROSCLEROSIS; PLASMA; GLUTATHIONE; OXIDE;
Keywords:
oxysterol; 7 beta-hydroxycholesterol; apoptosis; alpha-tocopherol; gamma-tocopherol; U937 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: O'Brien, NM Natl Univ Ireland Univ Coll Cork, Dept Food Sci Food Technol &Nutr, Cork,Ireland Natl Univ Ireland Univ Coll Cork Cork Ireland Cork,Ireland
Citazione:
N.M. Lyons et al., "alpha-Tocopherol, but not gamma-tocopherol inhibits 7 beta-hydroxycholesterol-induced apoptosis in human U937 cells", FREE RAD RE, 35(3), 2001, pp. 329-339

Abstract

Oxysterols, particularly those oxidised at position 7, are toxic to cells in culture and have been shown to induce apoptosis in cell types such as vascular endothelial cells, smooth muscle cells and monocytes. The precise mechanism by which oxysterols induce apoptosis is unknown but may involve thegeneration of oxidative stress. In the present study we examined the ability of alpha -TOC, alpha -TOC acetate (alpha -TOCA) and gamma -TOC to protect against 7 beta -hydroxycholesterol (7 beta -OHC)-induced apoptosis of human monocytic U937 cells. 7 beta -OHC is one of the most commonly detected oxysterols in foods and its level in plasma has been positively associated with an increased risk of atherosclerosis. The present study demonstrates a significant decrease in cell membrane integrity and cellular glutathione levels when U937 cells were treated with 30muM 7 beta -OHC. DNA fragmentation also occurred, as measured by agarose gel electrophoresis, and the number of apoptotic cells increased as assessedby nuclear morphology. Analysis by HPLC showed that there was a greater incorporation of gamma -TOC into U937 cells after a 48 h incubation, than either alpha -TOC or alpha -TOCA. However, despite the increased uptake of gamma -TOC, only alpha -TOC, and not gamma -TOC or alpha -TOCA was effective at inhibiting 7 beta -OHC-induced apoptosis in U937 cells.

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Documento generato il 14/07/20 alle ore 12:17:37