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Titolo:
An electron spin resonance study on alkylperoxyl radical in thin-sliced renal tissues from ferric nitrilotriacetate-treated rats: the effect of alpha-tocopherol feeding
Autore:
Toyokuni, S; Masumizu, T; Ozeki, M; Kondo, S; Hiroyasu, M; Kohno, M; Hiai, H;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Pathol & Biol Dis, Kyoto, Japan Kyoto UnivKyoto Japan ad Sch Med, Dept Pathol & Biol Dis, Kyoto, Japan JEOL Ltd, Analyt Instruments Div, Tokyo, Japan JEOL Ltd Tokyo JapanJEOL Ltd, Analyt Instruments Div, Tokyo, Japan
Titolo Testata:
FREE RADICAL RESEARCH
fascicolo: 3, volume: 35, anno: 2001,
pagine: 245 - 255
SICI:
1071-5762(2001)35:3<245:AESRSO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED LIPID-PEROXIDATION; OXIDATIVE STRESS; WISTAR RATS; CELL CARCINOMA; MOUSE KIDNEY; DAMAGE; CARCINOGENESIS; INDUCTION; CHROMATIN; NECROSIS;
Keywords:
electron spin resonance; tissue ESR; kidney; ferric nitrilotriacetate; peroxyl radical; alpha-tocopherol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Toyokuni, S Kyoto Univ, Grad Sch Med, Dept Pathol & Biol Dis, Kyoto, JapanKyoto Univ Kyoto Japan Dept Pathol & Biol Dis, Kyoto, Japan
Citazione:
S. Toyokuni et al., "An electron spin resonance study on alkylperoxyl radical in thin-sliced renal tissues from ferric nitrilotriacetate-treated rats: the effect of alpha-tocopherol feeding", FREE RAD RE, 35(3), 2001, pp. 245-255

Abstract

Formation of excess free radical causes cellular oxidative stress, which has been shown to be associated with a variety of pathologic conditions. While electron spin resonance (ESR) spectroscopy has been the only method to demonstrate the presence of free radicals, its application to tissue sampleshas been challenging. We report here the successful ESR detection in thin-sliced fresh tissues or frozen sections in a rat model. Ferric nitrilotriacetate (Fe-NTA) induces oxidative renal tubular damage that ultimately leadsto high incidence of renal carcinoma in rodents. Twenty minutes after administration of 5 mg iron/kg Fe-NTA to rats, a thin-slice of the kidney was mounted on a tissue-type cell and analyzed by ESR spin trapping with 5,5-dimethyl-1-pyrroline-N-oxide (DNPO). An ESR signal from alkylperoxyl radical adduct was obtained, and the signal was inversely proportional to renal alpha -tocopherol content which was modulated through diet. Furthermore, we undertook ex vivo study using frozen sections. Fe-NTA (1 mM) was added to a rat kidney frozen section for 10 min. After washing the specimen was mounted on a tissue-type cell and analyzed with ESR spin trapping using DMPO. Alkylperoxyl radical signal was dependent on thickness, incubation time and renal tissue levels of a-tocopherol, and was reduced by preincubation with catalase or dimethyl sulfoxide but not with ce-tocopherol outside tissue. This versatile method facilitates identification of free radicals in pathologic conditions, and may be useful for selection of antioxidants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:59:15