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Titolo:
Use of a green fluorescent protein gene as a reporter in Zymomonas mobilisand Halomonas elongata
Autore:
Douka, E; Christogianni, A; Koukkou, AI; Afendra, AS; Drainas, C;
Indirizzi:
Univ Ioannina, Dept Chem, Sector Organ Chem & Biochem, GR-45110 Ioannina, Greece Univ Ioannina Ioannina Greece GR-45110 iochem, GR-45110 Ioannina, Greece
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 2, volume: 201, anno: 2001,
pagine: 221 - 227
SICI:
0378-1097(20010724)201:2<221:UOAGFP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ICE NUCLEATION GENE; PSEUDOMONAS-SYRINGAE; EXPRESSION; PLASMID; IDENTIFICATION; CONSTRUCTION; REPLICATION; BACTERIA; FUSIONS; BIOLOGY;
Keywords:
green fluorescent protein; reporter gene; Zymomonas mobilis; Halomonas elongata;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Drainas, C Univ Ioannina, Dept Chem, Sector Organ Chem & Biochem, GR-45110Ioannina, Greece Univ Ioannina Ioannina Greece GR-45110 45110 Ioannina, Greece
Citazione:
E. Douka et al., "Use of a green fluorescent protein gene as a reporter in Zymomonas mobilisand Halomonas elongata", FEMS MICROB, 201(2), 2001, pp. 221-227

Abstract

We investigated the applicability of the green fluorescent protein of Aequorea victoria as a reporter for gene expression in the strictly fermentative Gram-negative ethanologenic bacterium Zymomonas mobilis and in the moderately halophilic bacterium Halomonas elongata. We have succeeded to express a mutated gene of green fluorescent protein under the control of different promoters in Z. mobilis and H. elongata grown under various glucose or saltconcentrations, respectively. Our results demonstrate that gfp can serve as an easily assayable reporter gene in both organisms. Maximum fluorescencewas obtained in Z. mobilis grown aerobically and in H. elongata grown under elevated salt concentration in solid medium. For both bacteria the fluorescence obtained was higher when the gf gene was placed under the control ofa native promoter. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V.. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 20:56:32