Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Lymphocyte population and apoptosis in the lungs of smokers and their relation to emphysema
Autore:
Majo, J; Ghezzo, H; Cosio, MG;
Indirizzi:
McGill Univ, Royal Victoria Hosp, MUHC Resp Div, Montreal, PQ H3A 1A1, Canada McGill Univ Montreal PQ Canada H3A 1A1 Div, Montreal, PQ H3A 1A1, Canada Univ Barcelona, Hosp Gen Valle Hebron, Dept Anat Patol, Barcelona, Spain Univ Barcelona Barcelona Spain ebron, Dept Anat Patol, Barcelona, Spain McGill Univ, Meakins Christie Labs, Montreal, PQ, Canada McGill Univ Montreal PQ Canada akins Christie Labs, Montreal, PQ, Canada
Titolo Testata:
EUROPEAN RESPIRATORY JOURNAL
fascicolo: 5, volume: 17, anno: 2001,
pagine: 946 - 953
SICI:
0903-1936(200105)17:5<946:LPAAIT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
OBSTRUCTIVE PULMONARY-DISEASE; CIGARETTE SMOKERS; T-LYMPHOCYTES; DESTRUCTION; DEFICIENCY; SMOKING; BRONCHOALVEOLAR; RECOGNITION; AIRWAYS; CELLS;
Keywords:
apoptosis; CD8+; chronic obstructive pulmonary disease; emphysema; T-cells; T-lymphocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Cosio, MG McGill Univ, Royal Victoria Hosp, MUHC Resp Div, 687 Pine Ave W,Montreal,PQ H3A 1A1, Canada McGill Univ 687 Pine Ave W Montreal PQ Canada H3A 1A1 A1, Canada
Citazione:
J. Majo et al., "Lymphocyte population and apoptosis in the lungs of smokers and their relation to emphysema", EUR RESP J, 17(5), 2001, pp. 946-953

Abstract

Previously, it had been shown that T-lymphocytes are the predominant inflammatory cells found in the alveolar wall of smokers and their numbers correlated with the extent of emphysema. However, the phenotype of these cells was not defined. The aim of this study was to describe the different T-cell phenotypes and investigate the possible presence of apoptosis in the lung parenchyma of smokers. Samples from lungs were obtained at surgery from 15 patients who smoked and six who had never smoked. Samples were frozen and prepared for histological and immunocytochemical examination. Slides were stained for CD3+, CD4+, CD8+, gamma delta T-cells, CD56 natural killers ((INK) cells), and elastase(neutrophils). Anti-CD95 monoclonal antibodies and in situ end-labelling techniques were used to detect Fas expression and apoptosis. Positive staining cells were expressed as cells-mm alveolar wall(-1), percentage of total cells, and Fas/APO and apoptosis index. Emphysema was identified macroscopically, microscopically and reported as present or absent. All subjects had pulmonary function tests before surgery. Neutrophils were the predominant cell in the lung parenchyma of nonsmokersand smokers without emphysema. In smokers with emphysema, the CD3+ and CD8 were the predominant cells (p <0.05) in the alveolar wall. gamma delta cells were increased in all smokers and no increased numbers of NK cells was found. The T-cell numbers-min alveolar wall(-1) showed a bilinear relationship with the amount smoked increasing at an 2 inflection point of 30 packs yr(-1) (R-2 = 0.345; p < 0.01). Apoptosis in smokers showed a bilinear relationship with the amount smoked increasing sharply in smokers with emphysema (R-2 = 0.3613; p < 0.009). It is concluded that the pathogenesis of emphysema might be mediated by T-lymphocytes. mainly CD8+ cytolytic T-cells, and that apoptosis might be oneof the mechanisms of lung destruction leading to the development of emphysema. If this is the case, it could be speculated that T-cell inflammation is a response to antigenic stimuli originating in the lung and induced by cigarette smoking.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 21:54:41