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Titolo:
Assessment of chloroaniline toxicity by the submitochondrial particle assay
Autore:
Argese, E; Bettiol, C; Agnoli, F; Zambon, A; Mazzola, M; Ghirardini, AV;
Indirizzi:
Univ Venice, Dept Environm Sci, I-30123 Venice, Italy Univ Venice VeniceItaly I-30123 ept Environm Sci, I-30123 Venice, Italy
Titolo Testata:
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
fascicolo: 4, volume: 20, anno: 2001,
pagine: 826 - 832
SICI:
0730-7268(200104)20:4<826:AOCTBT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASSIFYING ENVIRONMENTAL-POLLUTANTS; OXIDATIVE-PHOSPHORYLATION; CHEMICAL DESCRIPTORS; MITOCHONDRIA; CHLOROPHENOLS; UNCOUPLERS; MECHANISM; RESPONSES; SYSTEM; QSAR;
Keywords:
submitochondrial particle; bioassay; chloroaniline; toxicity; quantitative structure-activity relationships;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Argese, E Univ Venice, Dept Environm Sci, Calle Larga S Marta 2137, I-30123 Venice, Italy Univ Venice Calle Larga S Marta 2137 Venice Italy I-30123 Italy
Citazione:
E. Argese et al., "Assessment of chloroaniline toxicity by the submitochondrial particle assay", ENV TOX CH, 20(4), 2001, pp. 826-832

Abstract

The effects on mitochondrial respiration of 15 chloroanilines were recorded by using the in vitro response of submitochondrial particles (SMP) from beef heart mitochondria. The bioassay procedure for SMP is based on the process of reverse electron transfer, which can be negatively affected by inhibitors of electron transport, by uncouplers, and by chemicals that impair membrane integrity. The EC50 values, determined for the tested chloroanilines, indicate a general tendency of increasing toxicity with increasing chlorine substitution. In order to validate the results obtained and to evaluate the capability of the SMP assay to reproduce the toxic effects of the examined compounds on different freshwater species, the EC50 values were compared with literature data from other biological assays regarding both in vitrosystems and whole organisms. A good correlation was found in particular with two widely used testing systems, the Microtox((R)) and the Tetrahymena assays. In addition, quantitative structure-activity relationships (QSARs) were established between the EC50 values and various molecular descriptors for hydrophobic, steric, and electronic interactions. The results obtained were utilized to elucidate the mechanism of toxic action of chloroanilines, which are commonly reported to act by the polar narcosis mode of action. Moreover, they confirmed that the SMP assay can be a useful tool for studyingthe toxicity of chemicals that act nonspecifically by impairing membrane structure and functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 17:56:30