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Titolo:
Relative potencies of polychlorinated dibenzo-p-dioxin, dibenzofuran, and biphenyl congeners to induce cytochrome P4501A mRNA in a zebrafish liver cell line
Autore:
Henry, TR; Nesbit, DJ; Heideman, W; Peterson, RE;
Indirizzi:
US EPA, Natl Hlth & Environm Effects Res Lab, Duluth, MN 55804 USA US EPADuluth MN USA 55804 Environm Effects Res Lab, Duluth, MN 55804 USA Univ Wisconsin, Sch Pharm, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 sin, Sch Pharm, Madison, WI 53706 USA Univ Wisconsin, Ctr Environm Toxicol, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 vironm Toxicol, Madison, WI 53706 USA
Titolo Testata:
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
fascicolo: 5, volume: 20, anno: 2001,
pagine: 1053 - 1058
SICI:
0730-7268(200105)20:5<1053:RPOPDD>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOXIC EQUIVALENCY FACTORS; HALOGENATED AROMATIC-HYDROCARBONS; TROUT ONCORHYNCHUS-MYKISS; LIFE-STAGE DEVELOPMENT; CARP CYPRINUS-CARPIO; RAINBOW-TROUT; RECEPTOR AGONISTS; 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN; PCBS; FISH;
Keywords:
dioxin; toxicity equivalence factors; zebrafish; liver; cytochrome P4501A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Henry, TR US EPA, Natl Hlth & Environm Effects Res Lab, 6201 Congdon Blvd,Duluth, MN 55804 USA US EPA 6201 Congdon Blvd Duluth MN USA 55804 uluth, MN 55804 USA
Citazione:
T.R. Henry et al., "Relative potencies of polychlorinated dibenzo-p-dioxin, dibenzofuran, and biphenyl congeners to induce cytochrome P4501A mRNA in a zebrafish liver cell line", ENV TOX CH, 20(5), 2001, pp. 1053-1058

Abstract

Induction of cytochrome P4501A (CYP1A) mRNA by polychlorinated dibenzo-p-dioxin (PCDD), polychlorinated dibenzofuran (PCDF), and polychlorinated biphenyl (PCB) congeners was measured in a zebrafish liver (ZF-L) cell line. The ZF-L cells were far less sensitive to PCDD, PCDF, and PCB congeners than were other fish cell lines. The 2,3,7,8-PCDDs, 2,3,7,8-PCDFs, and PCB 126 caused dose-related induction. All other PCBs tested, including other coplanar as well as ortho-substituted congeners, were ineffective at inducing CYP1A. The potency of each congener that gave a response, relative to 2,3,7,8-tetrachlorodibenzo-p-dioxin, was determined. The ZF-L cell-derived relativepotency values (REPs) are similar to other in vitro REPs in that the ZF-L cell-derived REPs are generally higher than those derived from in vivo models. Furthermore, the ZF-L cell-derived REPs are generally within fivefold of REPs determined in a variety of rainbow trout systems when the same endpoint in the same tissue are compared. Analysis of these data indicates that REPs based on molecular and biochemical responses in sensitive and insensitive species are similar, but overestimate relative in vivo toxicity in the rainbow trout. The ZF-L cell-derived REPs expand the database of REPs, providing additional information that will be useful in quantifying the uncertainty associated with applying consensus fish-specific toxic equivalency factors in ecological risk assessment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:46:45