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Titolo:
Monitoring of endocrine disruptors in surface waters by the yeast recombinant assay
Autore:
Garcia-Reyero, N; Grau, E; Castillo, M; De Alda, MJL; Barcelo, D; Pina, B;
Indirizzi:
CSIC, Inst Biol Mol Barcelona, ES-08034 Barcelona, Spain CSIC Barcelona Spain ES-08034 l Mol Barcelona, ES-08034 Barcelona, Spain CSIC, Inst Invest Quim & Ambientales, ES-08034 Barcelona, Spain CSIC Barcelona Spain ES-08034 m & Ambientales, ES-08034 Barcelona, Spain
Titolo Testata:
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
fascicolo: 6, volume: 20, anno: 2001,
pagine: 1152 - 1158
SICI:
0730-7268(200106)20:6<1152:MOEDIS>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ESTROGEN-RECEPTOR; ANTIESTROGENIC ACTIVITY; ENVIRONMENTAL-SAMPLES; TRANSCRIPTION; NONYLPHENOL; ACTIVATION; TROUT; IDENTIFICATION; CEREVISIAE; COMPONENTS;
Keywords:
yeast bioassay; water pollution; polar surfactants; estrogens; hormone receptors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Pina, B CSIC, Inst Biol Mol Barcelona, ES-08034 Barcelona, Spain CSIC Barcelona Spain ES-08034 rcelona, ES-08034 Barcelona, Spain
Citazione:
N. Garcia-Reyero et al., "Monitoring of endocrine disruptors in surface waters by the yeast recombinant assay", ENV TOX CH, 20(6), 2001, pp. 1152-1158

Abstract

Endocrine disruptors exert physiological effects at very low concentrations. Surface waters present often a mixture of high concentrations of low-potency disruptors and low amounts of very powerful ones, making their chemical analysis complicated and expensive. We developed a recombinant yeast assay (RYA) for estrogenic Compounds using 96-well microtiter plates. This assay is based on three yeast strains, transformed with self-propagating plasmids. One strain contains an expression plasmid for the human estrogen hormone receptor and an appropriate reporter; it detects estrogenic and antiestrogenic activities. The two other yeast strains, one expressing the human progesterone receptor and a second based on the yeast activator Ga14p, served to analyze the nature of antiestrogenic activities. We applied this technique to water samples from two tributaries of the Llobregat river (NE Spain) as well as from four sewage treatment plants discharging on them. Our results indicate that the efficiency of sewage treatment plants for eliminating estrogenic compounds varied notably, being in at least one case completely inefficient. We also observed a prevalence of an inhibitory activity all through the two rivers; this inhibition was hormone specific. These results were consistent to previously obtained chemical analyses of the same samples, although chemical and in vivo analyses showed rather different levels of sensitivity for some compounds. Our findings demonstrate the utility of theyeast recombinant assay for analyzing complex natural samples; at the sametime, they stress the necessity of a panel of different yeast systems to adequately describe endocrine-disruptor activities.

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Documento generato il 11/07/20 alle ore 04:42:43