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Titolo:
Capillary electrophoresis of high-molecular chitosan: The natural carbohydrate biopolymer
Autore:
Ban, E; Choi, OK; Ryu, JC; Yoo, YS;
Indirizzi:
Korea Inst Sci & Technol, Bioanal & Biotransformat Res Ctr, Seoul 130650, South Korea Korea Inst Sci & Technol Seoul South Korea 130650 ul 130650, South Korea Dongyoung Inst Nonhyun Dong, Seoul, South Korea Dongyoung Inst Nonhyun Dong Seoul South Korea Dong, Seoul, South Korea
Titolo Testata:
ELECTROPHORESIS
fascicolo: 11, volume: 22, anno: 2001,
pagine: 2217 - 2221
SICI:
0173-0835(200107)22:11<2217:CEOHCT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-LINKED OLIGOSACCHARIDE; SOLUBLE CHITOSAN; CHROMATOGRAPHY; DEACETYLATION; SEPARATION; ACID;
Keywords:
chitosan; capillary electrophoresis; carbohydrates; polysaccharides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Yoo, YS Korea Inst Sci & Technol, Bioanal & Biotransformat Res Ctr, POB 131, Seoul130650, South Korea Korea Inst Sci & Technol POB 131 Seoul South Korea 130650 h Korea
Citazione:
E. Ban et al., "Capillary electrophoresis of high-molecular chitosan: The natural carbohydrate biopolymer", ELECTROPHOR, 22(11), 2001, pp. 2217-2221

Abstract

Due to its high resolving power and diverse application range, capillary electrophoresis (CE) has been successfully applied to the analysis of carbohydrates. In this paper, a method for the determination of high-molecular chitosan (M-r 200 000) using CE is presented. We studied the optimal condition of buffer pH and type, and column type for determination of chitosan. Optimal CE performance was found when employing 100 mM triethylamine (TEA)-phosphate buffer, pH 2.0 and untreated fused-silica capillary (50 mum x 27 cm)for the chitosan analysis. Under optimum conditions, excellent linear responses were obtained in the concentration range of 1.25-20 mum, with a linear correlation coefficient of 0.9983. The standard deviations of the migration time and peak area were found to be 2.5 and 6.4%, respectively. This method could be readily applied to chitosan determination in real biological samples and commercial products.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 12:16:57