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Titolo:
Hepatic tissue engineering on 3-dimensional biodegradable polymers within a pulsatile flow bioreactor
Autore:
Torok, E; Pollock, JM; Ma, PX; Vogel, C; Dandri, M; Petersen, J; Burda, MR; Kaufman, PM; Kluth, D; Rogiers, X;
Indirizzi:
Univ Hamburg, Dept Hepatobiliary Surg, Hamburg, Germany Univ Hamburg Hamburg Germany Dept Hepatobiliary Surg, Hamburg, Germany Univ Michigan, Sch Dent, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 n, Sch Dent, Ann Arbor, MI 48109 USA Univ Hamburg, Heinrich Pette Inst, Hamburg, Germany Univ Hamburg HamburgGermany urg, Heinrich Pette Inst, Hamburg, Germany Univ Hamburg, Dept Pediat Surg, Hamburg, Germany Univ Hamburg Hamburg Germany amburg, Dept Pediat Surg, Hamburg, Germany Univ Hamburg, Dept Internal Med, Hamburg, Germany Univ Hamburg Hamburg Germany mburg, Dept Internal Med, Hamburg, Germany
Titolo Testata:
DIGESTIVE SURGERY
fascicolo: 3, volume: 18, anno: 2001,
pagine: 196 - 203
SICI:
0253-4886(2001)18:3<196:HTEO3B>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRAPORTAL HEPATOCYTE TRANSPLANTATION; VIVO GENE-THERAPY; HOMOZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA; ADULT-RAT HEPATOCYTES; LONG-TERM CULTURE; SPHEROID FORMATION; EXTRACELLULAR-MATRIX; SYNTHETIC-POLYMER; LIVER; CELL;
Keywords:
tissue engineering; spheroid; hepatocyte transplantation; flow culture; bioreactor; albumin ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Torok, E Univ Hamburg, Hosp Eppendorf, Martinistr 52, D-20246 Hamburg, Germany Univ Hamburg Martinistr 52 Hamburg Germany D-20246 burg, Germany
Citazione:
E. Torok et al., "Hepatic tissue engineering on 3-dimensional biodegradable polymers within a pulsatile flow bioreactor", DIGEST SURG, 18(3), 2001, pp. 196-203

Abstract

Background: An optimal method for hepatocyte transplantation is not yet determined. With the principles of tissue engineering in vitro conditioning of hepatocytes on biodegradable polymer in a flow bioreactor before implantation forming spheroids may achieve increased cell mass and function to replace lost organ function in vivo. Methods: Biodegradable poly-L-lactic (PLLA) polymer discs were seeded with rat hepatocytes in a concentration of 10 x10(6) cells per ml and exposed to a medium flow of 24 ml/min for 1, 2, 4 and 6 days. The number and diameter of spheroidal aggregates was measured byphase-contrast microscopy. H&E histology was performed. Albumin productionas hepatocyte specific function was determined by ELISA. Results: Spheroids of viable hepatocytes of 50-200 mum in diameter were formed. Both the number and diameter of the spheroids increased during the first 2 days and then remained constant until day 6. Albumin production was maintained throughout the culture period. Conclusion: Short (2-3 days) pre-transplant conditioning of hepatocytes in a flow bioreactor on biodegradable PLLA resulted in formation of spheroids with a liver-like morphology and preserved specific metabolic function. Tissue engineered hepatocyte spheroids on polymer may represent a functionally active and easy transplantable neotissue and may serve as an in vivo substitute for lost liver function. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 00:34:25