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Titolo:
Neonatal estrogen exposure inhibits steroidogenesis in the developing rat ovary
Autore:
Ikeda, Y; Nagai, A; Ikeda, MA; Hayashi, S;
Indirizzi:
Univ Tsukuba, Inst Basic Med Sci, Dept Anat, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan Tokyo Med & Dent Univ, Grad Sch, Sect Mol Embryol, Bunkyo Ku, Tokyo, JapanTokyo Med & Dent Univ Tokyo Japan Mol Embryol, Bunkyo Ku, Tokyo, Japan Yokohama City Univ, Grad Sch Integrated Sci, Kanazawa Ku, Yokohama, Kanagawa, Japan Yokohama City Univ Yokohama Kanagawa Japan Ku, Yokohama, Kanagawa, Japan
Titolo Testata:
DEVELOPMENTAL DYNAMICS
fascicolo: 4, volume: 221, anno: 2001,
pagine: 443 - 453
SICI:
1058-8388(200108)221:4<443:NEEISI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE REGULATORY PROTEIN; VENTROMEDIAL HYPOTHALAMIC NUCLEUS; GENE-EXPRESSION; RECEPTOR-BETA; FACTOR-I; TRANSCRIPTIONAL REGULATION; INDUCED CRYPTORCHIDISM; MOLECULAR-CLONING; GRANULOSA-CELLS; STAR GENE;
Keywords:
steroidogenic factor 1; steroidogenic acute regulatory protein; cholesterol side-chain cleavage cytochrome P450; estradiol benzoate (EB); estrogen receptor; rat ovary;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Ikeda, Y Univ Tsukuba, Inst Basic Med Sci, Dept Anat, 1-1-1 Tennohdai, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba 1-1-1 Tennohdai Tsukuba Ibaraki Japan 3058575 Japan
Citazione:
Y. Ikeda et al., "Neonatal estrogen exposure inhibits steroidogenesis in the developing rat ovary", DEV DYNAM, 221(4), 2001, pp. 443-453

Abstract

Treatment of newborn female rats with estrogens significantly inhibits thegrowth and differentiation of the ovary. To understand the molecular mechanism of estrogen action in the induction of abnormal ovary, we examined theexpression profiles of steroidogenic factor 1 (SF-1) and several of its target genes in the developing ovaries after neonatal exposure to synthetic estrogen, estradiol benzoate (EB) by using reverse transcriptase polymerase chain reaction, in situ hybridization, and immunohistochemistry. Morphologic examination indicated inhibitory effects of estrogen on the stratification of follicles and development of theca and interstitial gland during postnatal ovarian differentiation. The expression of the steroidogenic acute regulatory protein (StAR) and cholesterol side-chain cleavage cytochrome P450 (P450(SCC)), which are both essential for steroid biosynthesis, markedly decreased in theca and interstitial cells throughout the postnatal development of the EB-treated ovary. However, expression of the transcriptional activator of the two genes, SF-1 was unaffected in theca and interstitial cells,although the number of these cells was lower in the EB-treated ovary than in the control ovary. The expression of the estrogen mediator, estrogen receptor-alpha (ER-alpha), diminished specifically in theca cells at P6 and recovered by P14 in the EB-treated ovary. These results indicate that the effect of estrogens is mediated by means of ER-alpha resulting in the down-regulation of StAR and P450(SCC) genes during early postnatal development of the ovary. These results suggest that the abnormal ovarian development by neonatal estrogen treatment is closely correlated with the reduced steroidogenic activity, and the data obtained by using this animal model may account in part the mechanism for aberrant development and function of the ovary inprenatally estrogen-exposed humans. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 07:44:48