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Titolo:
Vascular smooth muscle cell phenotypic modulation in culture is associatedwith reorganisation of contractile and cytoskeletal proteins
Autore:
Worth, NF; Rolfe, BE; Song, J; Campbell, GR;
Indirizzi:
Univ Queensland, Dept Anat Sci, Ctr Res Vasc Biol, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 Brisbane, Qld 4072, Australia
Titolo Testata:
CELL MOTILITY AND THE CYTOSKELETON
fascicolo: 3, volume: 49, anno: 2001,
pagine: 130 - 145
SICI:
0886-1544(200107)49:3<130:VSMCPM>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA LOCALIZATION; HUMAN CAROTID ARTERIES; HEAVY-CHAIN ISOFORMS; ACTIN STRESS FIBERS; INTERMEDIATE FILAMENTS; ALPHA-ACTININ; FOCAL ADHESIONS; VIMENTIN FILAMENTS; NONMUSCLE ACTINS; EXPRESSION;
Keywords:
smooth muscle cell; phenotype; cytoskeleton; contractile proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Rolfe, BE Univ Queensland, Dept Anat Sci, Ctr Res Vasc Biol, Brisbane, Qld4072, Australia Univ Queensland Brisbane Qld Australia 4072 Qld 4072, Australia
Citazione:
N.F. Worth et al., "Vascular smooth muscle cell phenotypic modulation in culture is associatedwith reorganisation of contractile and cytoskeletal proteins", CELL MOTIL, 49(3), 2001, pp. 130-145

Abstract

Smooth muscle cells (SMC) exhibit a functional plasticity, modulating fromthe mature phenotype in which the primary function is contraction, to a less differentiated state with increased capacities for motility, protein synthesis, and proliferation. The present study determined, using Western analysis, double-label immunofluorescence and confocal microscopy, whether changes in phenotypic expression of rabbit aortic SMC in culture could be correlated with alterations in expression and distribution of structural proteins. "Contractile" state SMC (days 1 and 3 of primary culture) showed distinct sorting of proteins into subcellular domains, consistent with the theory that the SMC structural machinery is compartmentalised within the cell. Proteins specialised for contraction (alpha -SM actin, SM-MHC, and calponin) were highly expressed in these cells and concentrated in the upper central region of the cell. Vimentin was confined to the body of the cell, providingsupport for the contractile apparatus but not co-localising with it. In line with its role in cell attachment and motility, beta -NM actin was localised to the cell periphery and basal cortex. The dense body protein alpha -actinin was concentrated at the cell periphery, possibly stabilising both contractile and motile apparatus. Vinculin-containing focal adhesions were well developed, indicating the cells' strong adhesion to substrate. In "synthetic" state SMC (passages 2-3 of culture), there was decreased expression of contractile and adhesion (vinculin) proteins with a concomitant increase in cytoskeletal proteins (beta -non-muscle [NM] actin and vimentin). These quantitative changes in structural proteins were associated with dramatic chan-es in their distribution. The distinct compartmentalisation of structural proteins observed in "contractile" state SMC was no longer obvious, withproteins more evenly distributed throughout die cytoplasm to accommodate altered cell function. Thus, SMC phenotypic modulation involves not only quantitative changes in contractile and cytoskeletal proteins, but also reorganisation of these proteins. Since the cytoskeleton acts as a spatial regulator of intracellular signalling, reorganisation of the cytoskeleton may lead to realignment of signalling molecules, which, in turn, may mediate the changes in function associated with SMC phenotypic modulation. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 13:02:53