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Titolo:
Cell proliferation in the growing human heart: MIB-1 immunostaining in preterm and term infants at autopsy
Autore:
Huttenbach, Y; Ostrowski, ML; Thaller, D; Kim, HS;
Indirizzi:
Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 , Dept Pathol, Houston, TX 77030 USA
Titolo Testata:
CARDIOVASCULAR PATHOLOGY
fascicolo: 3, volume: 10, anno: 2001,
pagine: 119 - 123
SICI:
1054-8807(200105/06)10:3<119:CPITGH>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODY KI-67; CARDIAC MYOCYTES; NUCLEAR ANTIGEN; HYPERTROPHY; GROWTH; RAT; HYPERPLASIA; FETAL; MYOCARDIUM; CYCLE;
Keywords:
myocyte proliferation; human heart; preterm and term infants; autopsy; Ki-67; MIB-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Huttenbach, Y Baylor Coll Med, Dept Pathol, 1 Baylor Plaza, Houston, TX 77030 USA Baylor Coll Med 1 Baylor Plaza Houston TX USA 77030 7030 USA
Citazione:
Y. Huttenbach et al., "Cell proliferation in the growing human heart: MIB-1 immunostaining in preterm and term infants at autopsy", CARDIO PATH, 10(3), 2001, pp. 119-123

Abstract

Few studies of human cardiac myocyte proliferation in the perinatal periodhave been conducted. We measured the proliferative activity of left ventricular myocytes in tissue obtained at autopsy in three surgically induced abortuses, 20 preterm infants with gestational ages ranging from 12 to 35 weeks, eight term infants with ages ranging from I day to 11 months, and five adults. The preterm. infants lived less than 24 h, thus simulating the in utero condition of developing hearts. To assess the proliferative activity of the myocytes, we measured immunoreactivity using the monoclonal antibody MIB-1 against the recombinant Ki-67 nuclear antigen. Immunostained sectionswere examined by light microscopy, and the results expressed as a stainingindex (SI) of 0-3, according to the percentage of positively stained myocyte nuclei. Myocyte proliferative activity remained constant during the early preterm. period and decreased in the late preterm and early postterm periods. Adult myocytes, regardless of cardiac weight, did not reveal proliferative activity as assessed by immunostaining. This proliferation pattern is consistent with findings in most earlier studies in animal models. (C) 2001Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/01/20 alle ore 19:54:14