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Titolo:
Effect of serum depletion on centrosome overduplication and death of humanpancreatic cancer cells after exposure to radiation
Autore:
Shono, M; Sato, N; Mizumoto, K; Minamishima, YA; Nakamura, M; Maehara, N; Urashima, T; Saimura, M; Qian, L; Nishio, S; Nagai, E; Tanaka, M;
Indirizzi:
Kyushu Univ, Grad Sch Med Sci, Dept Surg & Oncol, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 t Surg & Oncol, Fukuoka 8128582, Japan
Titolo Testata:
CANCER LETTERS
fascicolo: 1, volume: 170, anno: 2001,
pagine: 81 - 89
SICI:
0304-3835(20010910)170:1<81:EOSDOC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HAMSTER OVARY CELLS; INDUCED APOPTOSIS; HELA-CELLS; CYCLE; DUPLICATION; MICRONUCLEI; EVENTS; REPRODUCTION; IRRADIATION; ABSENCE;
Keywords:
radiation; serum depletion; centrosome; multinucleated cell; mitotic cell death;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Mizumoto, K Kyushu Univ, Grad Sch Med Sci, Dept Surg & Oncol, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ol, Fukuoka 8128582, Japan
Citazione:
M. Shono et al., "Effect of serum depletion on centrosome overduplication and death of humanpancreatic cancer cells after exposure to radiation", CANCER LETT, 170(1), 2001, pp. 81-89

Abstract

The tumor microenvironment is one of the key factors affecting the cellular response to radiation; however, the influence of serum concentration on tumor radiosensitivity remains poorly understood. We recently discovered that gamma -irradiation of tumor cells causes centrosome overduplication, which may lead to lethal nuclear fragmentation through the establishment of multipolar mitotic spindles. In the present study, we investigated the effect of serum depletion on radiation-induced cell death in relation to the centrosome dynamics in human pancreatic cancer cells. Exposure of Capan-1 cells to gamma -irradiation resulted in a time-dependent increase in cells containing multiple centrosomes in association with the appearance of mitotic cell death. Treatment of irradiated cells with serum depletion drastically accelerated centrosome overduplication and the formation of multipolar spindles, resulting in increased nuclear fragmentation and cell death. Cell cycle analysis of irradiated cultures revealed that the reduced serum level increased the population of cells arrested in the G2/M phase, which might be responsible for the abnormal centrosome accumulation. These findings suggest that serum concentration can influence radiation-induced cell killing through modulating cell cycle progression and possibly centrosome overduplication. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 03:38:20